Overview

The isolated hepatocyte preparation, employed in suspension or culture, is now firmly established as a major tool not only for the study of the liver but also for the investigation of a large number of aspects of biology common to many cell types. This book provides a comprehensive description of advances in methodology and biology pertaining to the isolated hepatocyte preparation, and offers an insight into the value of isolated hepatocytes for basic and applied studies. A particular feature is the series of major reviews on growth regulation by hormones and growth factors, cell cycle progression and active cell death. The book also assembles for the first time novel work on the use of hepatocytes for clinical applications in the fields of transplantation, gene therapy and the bioartificial liver. Another important feature is a compilation of methods for isolated hepatocyte preparation from animals other than the rat, and includes several accounts of the isolation of human hepatocytes, which is becoming an increasingly important technique.

Full Product Details

Author:   M. N. Berry ,  Anthony M. Edwards
Publisher:   Kluwer Academic Publishers
Imprint:   Kluwer Academic Publishers
Edition:   2000 ed.
Dimensions:   Width: 15.50cm , Height: 3.30cm , Length: 23.50cm
Weight:   2.310kg
ISBN:  

9780792361770

ISBN 10:   0792361776
Pages:   606
Publication Date:   31 December 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1. Methods and Biology.- I. Isolated hepatocytes: forty years on.- 2. Isolation and culture of human hepatocytes.- 3. Isolation and culture of porcine hepatocytes.- 4. Preparation and properties of isolated hepatocytes from sheep.- 5. Elasmobranch hepatocytes.- 6. Isolation and cultivation of teleost hepatocytes.- 7. Conditions for growth of hepatocytes in culture.- 8. Engineering of a miniature 3-D hepatic cell plate culture model.- 9. Freezing and hypothermic storage of porcine hepatocytes.- 10. Storage of isolated hepatocytes.- 11. Hepatocyte swelling: techniques and effects on metabolism.- 12. Permeabilisation of hepatocytes with ?-toxin.- 13. Perifused immobilised hepatocytes for metabolic studies.- 14. Small hepatocytes in primary cultures.- 2. Reviews of Hepatocyte Biology.- 15. Mechanisms of hepatocyte growth regulation by hormones and growth factors.- 16. Proliferative response and metabolic effects of growth factors in human hepatocytes.- 17. Growth control and cell cycle progression in cultured hepatocytes.- 18. Mechanisms of active cell death in isolated hepatocytes.- 19. The protective role of nitric oxide in hepatocytes during responses to inflammatory mediators and induction of apoptosis.- 20. Short-term regulation by insulin of glucose metabolism in isolated and cultured hepatocytes.- 21. Studies on mitochondrial-cytoplasmic interactions in isolated hepatocytes from fasted rats.- 3. Applications: Pharmacology and Toxicology.- 22. The importance of hepatocytes in drug metabolism studies: an industrial perspective.- 23. Human hepatocytes as an experimental system for the evaluation of xenobiotics.- 24. Constitutive expression of cytochromes P450 in rat hepatocyte culture.- 25. Optimising conditions for induction of cytochrome P450 in primary hepatocytecultures.- 26. The use of isolated hepatocytes to study the mechanisms of action of environmental contaminants.- 27. The use of isolated hepatocytes for the study of xenobiotic hepatotoxicity.- 4. Therapeutic Applications.- 28. Human hepatocyte transplantation: biology and therapy.- 29. Ex vivo hepatocyte-directed gene therapy.- 30. A review of bioartificial liver development from an artificial organ engineering perspective.- 31. Development and testing of a bioartificial liver.- 32. The Berlin extracorporeal liver support system.- 33. The bioartificial liver of the Academic Medical Center at Amsterdam.- 34. Performance of a flat membrane bioreactor utilizing porcine hepatocytes cultured in an extracellular matrix.

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