Overview

Full Product Details

Author:   J.M. Walker ,  W. Gaastra
Publisher:   Kluwer Academic Publishers Group
Imprint:   Kluwer Academic Publishers
Edition:   Softcover reprint of the original 1st ed. 1983
Dimensions:   Width: 15.50cm , Height: 1.70cm , Length: 23.50cm
Weight:   0.516kg
ISBN:  

9780709927556

ISBN 10:   070992755
Pages:   335
Publication Date:   30 April 1983
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

1. High Performance Liquid Chromatography of Proteins and Nucleic Acids.- 2. Analytical Electrophoretic Techniques in Protein Chemistry.- 3. Protein Blotting.- 4. The Production and Isolation of Peptides for Sequence Analysis.- 5. Protein and Peptide Sequence Determination.- 6. The Extraction and Fractionation of RNA.- 7. Protein Synthesis In Vitro.- 8. Labelling DNA In Vitro — Nick Translation.- 9. Synthesis of cDNA for Molecular Cloning.- 10. Plasmid Isolation.- 11. The Use of Restriction Endonucleases and T4 DNA Ligase.- 12. Molecular Cloning of cDNA: Bacterial Transformation and Screening of Transformants.- 13. The Use of Minicells and Maxicells to Detect the Expression of Cloned Genes.- 14. Gel Electrophoresis of DNA.- 15. Detection of Specific DNA Sequences — The Southern Blot.- 16. The Determination of DNA Sequences.- 17. The Use of Cosmids as Cloning Vehicles.- Notes on Contributors.

Reviews

This book will be a very useful addition to department libraries, but it should also be of interest to undergraduates and researchers entering molecular biology. It should serve as a useful introduction to the practicalities of the newer techniques, and to serve to stimulate a proper practical approach to the subject at undergraduate level. The Times Higher Education Supplement

This book will be a very useful addition to department libraries, but it should also be of interest to undergraduates and researchers entering molecular biology. It should serve as a useful introduction to the practicalities of the newer techniques, and to serve to stimulate a proper practical approach to the subject at undergraduate level. The Times Higher Education Supplement

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