Overview

Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Full Product Details

Author:   Pui-Yan Kwok
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   v. 212
Dimensions:   Width: 15.20cm , Height: 2.10cm , Length: 22.90cm
Weight:   0.001kg
ISBN:  

9780896039681

ISBN 10:   0896039684
Pages:   288
Publication Date:   07 October 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

SNPs: Why Do We Care?Lisa D. BrooksDenaturing High-Performance Liquid ChromatographyAndreas Premstaller and Peter J. OefnerSNP Detection and Allele Frequency Determination by SSCPTomoko Tahira, Akari Suzuki, Yoji Kukita, and Kenshi HayashiConformation-Sensitive Gel ElectrophoresisArupa GangulyDetection of Mutations in DNA by Solid-Phase Chemical Cleavage Method: A Simplified AssayChinh T. Bui, Jeffrey J. Babon, Andreana Lambrinakos, and Richard G. H. CottonSNP Discovery by Direct DNA SequencingPui-Yan Kwok and Shenghui DuanComputational SNP Discovery in DNA Sequence DataGabor T. MarthGenotyping SNPs With Molecular BeaconsSalvatore A. E. Marras, Fred Russell Kramer, and Sanjay TyagiSNP Genotyping by the 5'-Nuclease ReactionKenneth J. LivakGenotyping SNPs by Minisequencing Primer Extension Using Oligonucleotide MicroarraysKatarina Lindroos, Ulrika Liljedahl, and Ann-Christine SyvanenQuantitative Analysis of SNPs in Pooled DNA Samples by Solid-Phase MinisequencingCharlotta Olsson, Ulrika Liljedahl, and Ann-Christine SyvanenHomogeneous Primer Extension Assay With Fluorescence Polarization DetectionTony M. Hsu and Pui-Yan KwokPyrosequencing for SNP GenotypingMostafa RonaghiHomogeneous Allele-Specific PCR in SNP GenotypingSoren Germer and Russell HiguchiOligonucleotide Ligation AssayJonas Jarvius, Mats Nilsson, and Ulf LandegrenInvader Assay for SNP GenotypingVictor Lyamichev and Bruce NeriMALDI-TOF Mass Spectrometry-Based SNP GenotypingNiels Storm, Brigitte Darnhofer-Patel, Dirk van den Boom, and Charles P. RodiIndex

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