Overview

Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail.  The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.

Full Product Details

Author:   Sara Lindström ,  Helene Andersson-Svahn
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2012 ed.
Volume:   853
Dimensions:   Width: 17.80cm , Height: 2.00cm , Length: 25.40cm
Weight:   0.671kg
ISBN:  

9781617795664

ISBN 10:   1617795666
Pages:   238
Publication Date:   10 February 2012
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Introduction: Why Analyze Single Cells?.- Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview.- Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry.- Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts.- Single-Cell Culture in Microwells.- Iso-Dielectric Separation and Analysis of Cells.- Single Cell Electroporation Using Microfluidic Devices.- Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array.- Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay.- Droplet Microfluidics for Single-Cell Analysis.- Screening of Antigen-Specific Antibody Secreting Cells.- Analysis of Single Eukaryotic Cells Using Raman Tweezers.- Single-Cell Microinjection Technologies.- Ultrasonic Manipulation of Single Cells.- Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices.- Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses.

Reviews

Author Information

Tab Content 6

Author Website:  

Countries Available

All regions