Overview

This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.

Full Product Details

Author:   Jose L. Luque-Garcia
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2603
Weight:   0.765kg
ISBN:  

9781071628621

ISBN 10:   1071628623
Pages:   286
Publication Date:   13 November 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Dynamic SILAC to Determine Protein Turnover in Neurons and Glia             Aline R. Dörrbaum, Erin M. Schuman, and Julian D. Langer   2. SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe             Dorothea Anrather, Silvia Bagelova Polakova, Lubos Cipak, and Juraj Gregan   3. Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quantitative Proteomics             Monika Kopeckova, Marek Link, and Ivona Pavkova   4. pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles             So-Fong Cam Ngan, Neil E. McCarthy, and Siu Kwan Sze   5. Quantification of Protein Palmitoylation by Cysteine-SILAC             Caiyun Fang, Xiaoqin Zhang, and Haojie Lu   6. Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC)             Georgia Chachami and Sina-Victoria Barysch   7. A Super-SILAC Approach for Profiling Histone Post-Translational Modifications             Roberta Noberini, Elisa Longhi, and Tiziana Bonaldi   8. SILAC-Based Quantitative Phosphoproteomics in Yeast             María Luisa Hernáez and Concha Gil   9. Phosphotyrosine Profiling Using SILAC             Keshava K. Datta, Aditi Chatterjee, and Harsha Gowda   10. Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins             Rong Cai and Yinsheng Wang   11. Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactive Molecules             Tomonori Tamura and Itaru Hamachi   12. Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC             Alexis Gonneaud, Claude Asselin, Véronique Giroux, and François-Michel Boisvert   13. Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons             Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert   14. Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/K-Methylated Peptides by High-Resolution Mass Spectrometry             Enrico Massignani, Marianna Maniaci, and Tiziana Bonaldi   15. SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos             Alistair Langlands, Hamze Beati, and H.- Arno J. Müller   16. Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae             Guillermo Aragoneses-Cazorla, Andres Machuca, M. Pilar Buendia-Nacarino, Daniela S. Anunciação, Estefania Garcia-Calvo, and Jose L. Luque-Garcia   17. Use of Nuclear and Chromatin Enrichment Procedures for Quantitation of Yeast DNA Replication Proteins Using SILAC             Takashi Kubota   18. Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trapping for the Detection of Transient Protein Interactions             Jolene M. Duda and Stefani N. Thomas   19. Profiling of Secreted Proteins in Serum-Containing Media Using BONCAT and Pulsed SILAC             Jihye Shin and Cheolju Lee   20. Using SILAC to Develop Quantitative Data Independent Acquisition (DIA) Proteomics Methods             Ellen P. Casavant, Jason Liang, Sumedh Sankhe, W. Rodney Mathews, and Veronica G. Anania   21. SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status             Marie Vajrychova, Barbora  Salovska, Kristyna Pimkova, Ivo Fabrik, and Zdenek Hodny   22. Targeted Absolute Protein Quantification Using SILAC Internal Standard and Full-Length Protein Calibrators (TAQSI)             Xinwen Wang, Jian Shi, and Hao-Jie Zhu

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