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OverviewFull Product DetailsAuthor: Alexander E. KalyuzhnyPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 3rd ed. 2023 Volume: 2593 Weight: 0.838kg ISBN: 9781071628102ISBN 10: 1071628100 Pages: 328 Publication Date: 14 December 2022 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand We will order this item for you from a manufactured on demand supplier. Table of Contents1. Top 5 Considerations When Choosing an Antibody Amy Archuleta and Michael D. Browning 2. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Biji T. Kurien and R. Hal Scofield 3. Recent Advances in Chromogens for Immunohistochemistry Bipin Gupta, George Yang, Olga Petrauskene, and Marc Key 4. Overview of Imaging Techniques in Immunohistochemistry Gerald Sedgewick 5. Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells Pravin J. Mishra and Debabrata Banerjee 6. Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments Hemant K. Mishra 7. Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex Immunostaining Jingwen Niu, Jodi Hagen, Feifan, Yu, Alexander E. Kalyuzhny, and Andrew Tsourkas 8. Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species Matthew Levin, Zohreh AkhavanAghdam, and David Schwartz 9. 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes Sharla L. White, Amy T. Lam, and Hannah D. Buck 10. Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research Simeon C. Daeschler and Gregory H. Borschel 11. Protocol and Software for Automated Detection of Lysosome Active “Runs” and “Flights” with Wavelet Transform Approach Kristiana Kandere-Grzybowska, Konstantin Polev, Diana V. Kolygina, and Bartosz A. Grzybowski 12. Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing Christine Goetz, Chris Hammerbeck, Kristina Boss, Ryan Peterson, and Jody Bonnevier 13. In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells Jodi Hagen, Ashley Oliver, and Alexander E. Kalyuzhny 14. In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue Ashley Oliver, Jodi Hagen, Sue Yang, and Alexander E. Kalyuzhny 15. Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry Wenfei Kang, Anthony Santella, Eric Rosiek, Maria Pulina, Eric Chan, Ning Fan, Murray J. Tipping, Afsar Barlas, Yevgeniy Romin, and Katia Manova-Todorova 16. Transcription Factor-Mediated Differentiation of Motor Neurons from Human Pluripotent Stem Cells Junjiao Wu and Yu Tang 17. Immunohistochemical Techniques for Phosphoproteins Berenice L. Sanz Ressel and Alfredo A. Molinolo 18. Visualizing Cofilin-Actin Filaments by Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in Cells Laurie S. Minamide, Ryan Hylton, Matthew Swulius, and James R. Bamburg 19. Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Human Formalin-Fixed Paraffin-Embedded Brain Sections Clara Muñoz-Castro, Ayush Noori, Bradley T. Hyman, and Alberto Serrano-Pozo 20. Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry Lokman Pang, Matthias Ernst, and Jennifer Huynh 21. Highly Multiplexed Immuno-Fluorescence Imaging for Quantitative Spatial Analysis in Tissue Samples with ChipCytometryTM Thomas D. Campbell, Arne Christians, and Madison A. TylerReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |