Overview

This authoritative collection of basic and advanced protocols permits biotechnologists to use plants rather than single cell fermentation systems for expressing recombinant proteins. The protocols provide clear step-by-step instructions for the cloning, expression, and analysis of a wide variety of recombinant proteins in plants, and for the cloning of genes into a number of different plant species. The book also describes promising experimental techniques that will become increasingly important in the future, and includes techniques for the characterization of medically important proteins expressed in plants.

Full Product Details

Author:   Charles Cunningham ,  Andrew J. R. Porter
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1998 ed.
Volume:   3
Dimensions:   Width: 15.50cm , Height: 1.90cm , Length: 23.50cm
Weight:   0.631kg
ISBN:  

9780896033900

ISBN 10:   0896033902
Pages:   308
Publication Date:   24 December 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Rice Transformation by Agrobacterium Infection.- Barley as a Producer of Heterologous Protein.- Introducing and Expressing Genes in Legumes.- Use of the GUS Reporter Gene.- Expression of Recombinant Proteinase Inhibitors in Plants.- Detection of Recombinant Viral Coat Protein in Transgenic Plants.- Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants.- Tobamovirus Vectors for Expression of Recombinant Genes in Plants.- Single-Chain Fv Antibodies Expressed in Plants.- Characterization and Applications of Plant-Derived Recombinant Antibodies.- Production of Recombinant Antibodies in Plant Suspension Cultures.- Production of Foreign Proteins in Tobacco Cell Suspension Culture.- Transient Gene Expression in Plant Protoplasts.- Stability of Recombinant Proteins in Plants.- Trafficking and Stability of Heterologous Proteins in Transgenic Plants.- Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein.- Manipulation of Photosynthetic Metabolism.- Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA.- Analysis of N- and O-Glycosylation of Plant Proteins.- Two-Dimensional Electrophoresis.

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