Overview

An outstanding collection of state-of-the-art methods from leading experts for interrogating ligand-receptor and receptor-effector interactions. The methods emphasize the regulation of G protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G proteins, and approaches to studying how receptor expression and function is regulated. The easily reproducible protocols include chimeric receptor construction, antibody generation, transient and stable transfection methods, in situ hybridization, as well as immunocytochemistry, functional assays of G protein function, antisense methods for defining receptor-effector coupling pathways, and many more.

Full Product Details

Author:   R. A. J. Challiss
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1997 ed.
Volume:   83
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   1.340kg
ISBN:  

9780896034952

ISBN 10:   089603495
Pages:   275
Publication Date:   08 May 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of print, replaced by POD  
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Table of Contents

Methods for Characterizing Receptors.- Radioligand-Binding Methods for Membrane Preparations and Intact Cells.- Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding.- Approaches to the Stable Transfection of G Protein-Coupled Receptors.- Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels.- The Generation of Receptor-Selective Antibodies.- In Situ Hybridization.- lmmunocytochemical Methods for Investigating Receptor Localization.- Methods for Studying Receptor Coupling Mechanisms.- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Cell Membranes.- Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTP?S Binding.- Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes.- Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling.- Identification and Quantitation of G Protein ?-Subunits.- Covalent Modification of G Proteins by Affinity Labeling.- Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection.- Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identify G Protein Heterotrimers Activating Phospholipase C.- Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons.- Methods for Investigating the Regulation of Receptor Function.- Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation.- Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation.- Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cells.- Regulation of ReceptorExpression.

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