Overview

This book is the final part of a trilogy of volumes covering the practical aspects of ligand interactions, biochemistry, and effector mechanisms of signal-transducing receptors. The book focuses on the measurement of receptor binding interactions both at equilibrium and from the kinetic point of view. It includes descriptions of the most important techniques for the performance of ligand binding assays to both membrane-bound and solubilized receptors. In addition to which, a systematic effort has been made to provide insight into the strategy and tactics of receptor binding assays, and the conditions which must be satisfied if artifacts are to be avoided and interpretable data obtained. Since the behaviour of even the simplest receptor systems can be surprisingly complex, an attempt has been made to provide some computational tools which allow complications to be predicted and handled or avoided. Ligand binding assays are very widely used, not least in drug screening and development. This volume will help the practitioner to choose an appropriate ligand, make a suitable receptor preparation, design and perform a well thought out set of assays, and analyse the data obtained correctly.

Full Product Details

Author:   Hulme
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   92
Dimensions:   Width: 15.80cm , Height: 2.60cm , Length: 23.50cm
Weight:   0.734kg
ISBN:  

9780199630912

ISBN 10:   0199630917
Pages:   480
Publication Date:   07 May 1992
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Kevin G. McFarthing: Selection and synthesis of receptor-specific radioligands; A.D. Strosberg: Anti-receptor antibodies as ligands; J.O. Dolly: Neurotoxins as receptor ligands; E.C. Hulme & N.J.M. Birdsall: Strategy and tactics in receptor binding studies; E.C. Hulme & N.J. Buckley: Receptor preparations for binding studies; J-X. Wang, H.I. Yamamura, Wan Wang, & W.R. Roeske: The use of the filtration technique in In Vitro radioligand binding assays for membrane-bound and solubilised receptors; E.C. Hulme: Centrifugation binding assays; P.G. Strange: Charcoal adsorption for separating bound and free ligand in radioligand binding assays; E.C. Hulme: Gel filtration assays for solubilised receptors; H. Prinz: Receptor binding kinetics; J.W. Wells: Analysis and interpretation of binding data; Appendix 1: K.G. McFarthing: Radioligands for receptor binding: Amersham International; R.L.Young: Radiogands for receptor binding: Du Pont-New England Nuclear Corp.

Reviews

'This is an excellent multi-author book. The book represents very good value for the amount of information that it contains and will appeal to both the experienced and those starting receptor research. Selected chapters would be of relevance to undergraduates embarking on final year projects.' Anthony P. Davenport and Peter Molenaar, Addenbrookes Hospital, Trends in Biochemical Sciences, Volume 17 No. 12, December 1992 'a very timely publication ... This book, together with Receptor Biochemistry and Receptor-Effector Coupling, the other two books in this series, would form an invaluable resource for receptor studies.' A. Sivaprasadarao, Biomedical Education 21 (1) 1993 'a valuable bench manual for all researchers in biochemistry, molecular biology, neuroscience, and pharmacology who are interested in receptor function' Anticancer REsearch 12: (1992)

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