Overview

Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.

Full Product Details

Author:   Sharon Marsh
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2007 ed.
Volume:   373
Dimensions:   Width: 15.20cm , Height: 1.20cm , Length: 22.90cm
Weight:   1.050kg
ISBN:  

9781588296450

ISBN 10:   1588296458
Pages:   196
Publication Date:   21 November 2006
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

The History of Pyrosequencing®.- Pyrosequencing® Applications.- Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing®.- Pyrosequencing®.- Universal Primer Applications for Pyrosequencing®.- Allele Quantification and DNA Pooling Methods.- Multiplex Pyrosequencing® for DNA Variation Analysis.- Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing® Technology.- Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria.- Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci.- Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands.- Gene Copy Number Detection in Animal Studies.- Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing®.- Pyrosequencing® Genotype Storage Techniques.

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