Overview

This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest.  Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise. Authoritative and cutting-edge, Protein NMR: Methods and Protocol aims to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Ranajeet Ghose
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2018
Volume:   1688
Dimensions:   Width: 17.80cm , Height: 2.40cm , Length: 25.40cm
Weight:   0.868kg
ISBN:  

9781493984695

ISBN 10:   1493984691
Pages:   446
Publication Date:   23 May 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology Caitlin M. Quinn, Mingzhang Wang, and Tatyana Polenova   2. Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-state NMR of Microcrystalline and Membrane-Bound Proteins T. Gopinath and Gianluigi Veglia   3. Afterglow Solid-State NMR Spectroscopy Gili Abramov and Nathaniel J. Traaseth   4. Filamentous Bacteriophage Viruses: Preparation, Magic-angle Spinning Solid-state NMR Experiments and Structure Determination Omry Morag, Nikolaos G. Sgourakis, Gili Abramov, and Amir Goldbourt   5. Spherical Nanoparticle Supported Lipid Bilayers: A Tool for Modeling Protein Interactions with Curved Membranes Erin R. Tyndall and Fang Tian   6. Rapid Prediction of Multi-dimensional NMR Data Sets using FANDAS Siddarth Narasimhan, Deni Mance, Cecilia Pinto, Markus Weingarth, Alexandre M. J. J. Bonvin, and Marc Baldus   7. Strategies for Efficient Sample Preparation for Dynamic Nuclear Polar­ization Solid State NMR of Biological Macromolecules Boris Itin and Ivan V. Sergeyev   8. In-vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules Yaewon Kim, Yunyi Wang, Hsueh-Ying Chen, and Christian Hilty   9. Determination of Protein ps-ns Motions by High-Resolution Relaxometry Samuel F. Cousin, Pavel Kadeřávek, Nicolas Bolik-Coulon, and Fabien Ferrage   10. Characterizing Protein Dynamics with NMR R1r Relaxation Experiments Francesca Massi and Jeffrey W. Peng   11. CPMG Experiments for Protein Minor Conformer Structure Determination Anusha B Gopalan, D Flemming Hansen, and Pramodh Vallurupalli   12. Probing the Atomic Structure of Transient Protein Contacts by Paramagnetic Relaxation Enhancement Solution NMR Vincenzo Venditti and Nicolas L. Fawzi   13. From Raw Data to Protein Backbone Chemical Shifts Using NMRFx Processing and NMRViewJ Analysis Bruce A. Johnson   14. Protein Structure Elucidation from NMR Data with the Program Xplor-NIH Guillermo A. Bermejo and Charles D. Schwieters   15. Practical Nonuniform Sampling and Non-Fourier Spectral Reconstruction for Multidimensional NMR Mark W. Maciejewski, Adam D. Schuyler, and Jeffrey C. Hoch   16. Covariance NMR Processing and Analysis for Protein Assignment Bradley J. Harden and Dominique P. Frueh 17. Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System Wolfgang Peti, Rebecca Page, Evzen Boura, and Bartosz Rozycki   18. Implementation of the NMR CHEmical Shift Covariance Analysis (CHESCA): A Chemical Biologist’s Approach to Allostery Stephen Boulton, Rajeevan Selvaratnam, Rashik Ahmed, and Giuseppe Melacini   19. High-efficiency Expression of Yeast-derived G Protein-coupled Receptors and 19F Labeling for Dynamical Studies            Libin Ye, Alexander P. Orazietti, Aditya Pandey, and R. Scott Prosser   20. Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-cell NMR Spectroscopy David S. Burz, Christopher M. DeMott, Asma Aldousary, Stephen Danserea, and Alexander Shekhtman    

Reviews

The book covers current applications of solid-state and solution nuclear magnetic resonance spectroscopy to study protein structure, dynamics. and interactions. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols with tips to tackle troubleshooting. The book has several high quality illustrations in color or black and white. ... The intended audience appears to be students, specialists, and those who are interested in NMR spectroscopy. (Omer Iqbal, Doody's Book Reviews, April, 2018)

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