Principles of Light Microscopy: From Basic to Advanced

Author:   Volodymyr Nechyporuk-Zloy
Publisher:   Springer International Publishing AG
Edition:   1st ed. 2022
ISBN:  

9783031044762


Pages:   324
Publication Date:   30 November 2022
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Principles of Light Microscopy: From Basic to Advanced


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Overview

This textbook is an excellent guide to microscopy for students and scientists, who use microscopy as one of their primary research and analysis tool in the laboratory.  The book covers key microscopy principles and explains the various techniques such as epifluorescence microscopy, confocal/live cell imaging, SIM/light sheet microscopy, and many more. Easy-to-understand protocols provide helpful guidance for practical implementation in various commercially available imaging systems. The reader is introduced to histology and further be guided through advanced image acquisition, classification and analysis. The book is written by experienced imaging specialists from the UK, other EU countries, the US and Asia, and is based on advanced training courses for master students and PhD students. Readers are not expected to be familiar with imaging and microscopy technologies, but are introduced to the subject step by step. This textbook is indented for biomedical and medical students, as well as scientists and postdocs who want to acquire a thorough knowledge of microscopy, or gain a comprehensive overview of modern microscopy techniques used in various research laboratories and imaging facilities. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Full Product Details

Author:   Volodymyr Nechyporuk-Zloy
Publisher:   Springer International Publishing AG
Imprint:   Springer International Publishing AG
Edition:   1st ed. 2022
Weight:   0.516kg
ISBN:  

9783031044762


ISBN 10:   3031044762
Pages:   324
Publication Date:   30 November 2022
Audience:   College/higher education ,  Undergraduate
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Chapter 1. The physical principles of light propagation and light-matter interactions.- Chapter 2. Design, alignment and usage of infinity-corrected microscope.- Chapter 3. Epifluorescence Microscopy.- Chapter 4. Basic digital image design, processing, analysis, management, and presentation.-  Chapter 5. Confocal Microscopy.- Chapter 6. Live-cell Imaging: A Balancing Act Between Speed, Sensitivity and Resolution.-  Chapter 7. Structured Illumination Microscopy.- Chapter 8. Stimulated Emission Depletion Microscopy.-  Chapter 9. Two-Photon Imaging.- Chapter 10. Modern microscopy image analysis: quantifying colocalization on a mobile device.- Chapter 11. Spinning Disk Microscopy.-  Chapter 12. Light Sheet Fluorescence Microscopy.-  Chapter 13. Localization microscopy: a review of the progress in methods and applications.

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Author Information

Volodymyr Nechyporuk Zloy, PhD, is Bio Imaging Lab Manager at Nikon Europe, where his activities encompass the management of the equipment, the development of the new imaging services and education. Volodymyr has developed several microscopy courses for master and PhD students.

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