Overview

A unique collection of hands-on enzyme assay techniques to study polyamines and their function. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. The methods are presented by leading researchers who have perfected them to a high art, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.

Full Product Details

Author:   David M. L. Morgan
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   79
Dimensions:   Width: 15.50cm , Height: 1.80cm , Length: 23.30cm
Weight:   0.485kg
ISBN:  

9780896034488

ISBN 10:   0896034488
Pages:   186
Publication Date:   10 October 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Polyamines: An Introduction.- Assay Methods for Enzymes of Polyamine Biosynthesis.- Determination of Ornithine Decarboxylase Activity Using [3H]Omithine.- Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine.- Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity.- Assay of Spermidine and Spermine Synthases.- Measurement of Spermidine/Spermine N 1-Acetyltransferase Activity.- Assay of Spermidine N 8-Acetyltransferase.- Assay Methods for Enzymes of Polyamine Catabolism.- Assay of N 8-Acetylspermidine Deacetylase.- Hydrogen Peroxide Assay for Amine Oxidase Activity.- Radiochemical Assay of Diamine Oxidase.- Radiochemical Estimation of Polyamine Oxidase.- Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity.- Measurement of Polyamines.- Determination of Polyamines as Their Benzoylated Derivatives by HPLC.- A Dansyl Chloride-HPLC Method for the Determination of Polyamines.- The Determination of Polyamines and Amino Acids by a Fluorescamine-HPLC Method.- Thin-Layer Chromatographic Method for Assaying Polyamines.- Measurement of Polyamine Transport.- Measurement of Polyamine Transport: Adherent Cells.- Measurement of Polyamine Transport: Cells in Suspension.- Measurement of Polyamine Efflux from Cells in Culture.- Measurement of Polyamine Effects on Cell Growth.- Leucine Incorporation and Thymidine Incorporation.- Tetrazolium (MTT) Assay for Cellular Viability and Activity.

Reviews

This book would be a useful reference for groups already working in this area and an excellent starting point for those new to the subject. - Molecular Biotechnology

...This book would be a useful reference for groups already working in this area and an excellent starting point for those new to the subject. -Molecular Biotechnology The collection of gene therapy protocols is a valuable and indispensable resource for basic scientists, graduate students, and postdoctoral fellows. The book represents an outstanding work, which should not be missed in all laboratories dealing with gene therapy. - Neoplasma

...This book would be a useful reference for groups already working in this area and an excellent starting point for those new to the subject. -Molecular Biotechnology The collection of gene therapy protocols is a valuable and indispensable resource for basic scientists, graduate students, and postdoctoral fellows. The book represents an outstanding work, which should not be missed in all laboratories dealing with gene therapy. - Neoplasma

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