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OverviewFull Product DetailsAuthor: Tony Heitkam , Sònia GarciaPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 1st ed. 2023 Volume: 2672 Weight: 1.288kg ISBN: 9781071632253ISBN 10: 1071632256 Pages: 568 Publication Date: 20 June 2023 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand We will order this item for you from a manufactured on demand supplier. Table of ContentsPlant Cytogenetics: From Chromosomes to Cytogenomics.- The Use of Flow Cytometry for Estimating Genome Sizes and DNA Ploidy Levels in Plants.- Nuclear DNA Content Estimation of Seaweed by Fluorimetry Analysis.- K-Mer-Based Genome Size Estimation in Theory and Practice.- A Bioinformatic Pipeline to Estimate Ploidy Level from Target Capture Sequence Data Obtained from Herbarium Specimens.- Nitrous Oxide-Induced Metaphase Arrest: A Technique for Somatic Chromosome Analysis.- Preparation of Mitotic Chromosomes with the Squash Technique.- Preparation of Mitotic Chromosomes with the Dropping Technique.- Laser Capture Microdissection: From Genomes to Chromosomes, from Complex Tissue to Single-Cell Analysis.- Flow Cytometric Analysis and Sorting of Plant Chromosomes.- C-Banding of Plant Chromosomes.- CMA/DAPI Banding of Plant Chromosomes.- Silver Nitrate Staining of Nucleolar Organizer Regions (Ag-NORs) in Plant Chromosomes.- Chromatin Immunostaining of Plant Nuclei.- Critical Steps in DAPI and FISH Imaging of Chromosome Spread Preparations.- Formamide-Free Genomic In Situ Hybridization (ff-GISH).- The Use of Ribosomal DNA for Comparative Cytogenetics.- Identification of the Sequence and the Length of Telomere DNA.- Chromosome Painting Using Chromosome-Specific BAC Clones.- CRISPR-FISH: A CRISPR/Cas9-Based In Situ Labeling Method.- Preparation of Male Meiotic Chromosomes for Fluorescence In Situ Hybridization and Immunodetection with Major Focus on Dogroses.- Extended DNA Fibers for High-Resolution Mapping.- Visualizing Chromosome Territories and Nuclear Architecture of Large Plant Genomes Using Alien Introgressions.- Visualization of the Nucleolus Using 5´Ethynyl Uridine.- Bioinformatics Prediction of Bulked Oligonucleotide Probes for FISH Using Chorus2.- Visualization of Oligonucleotide-Based Probes along Pseudochromosomes Using RIdeogram, KaryoploteR, and Circlize (Circos).- Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding.- Flow Sorting-Assisted Optical Mapping.- Chromosome Conformation Capture of Mitotic Chromosomes.- Analysis of 5S rDNA Genomic Organization through the RepeatExplorer2 Pipeline: A Simplified Protocol.- Tools for Drawing Informative Idiograms.- Using ChromEvol to Determine the Mode of Chromosomal Evolution.- Online Resources Useful for Plant Cytogenetics and Cytogenomics Research.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |