Overview

Peter Eigil Nielsen has assembled a critically evaluated collection of key PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process.

Full Product Details

Author:   Peter E. Nielsen
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2002 ed.
Volume:   208
Dimensions:   Width: 14.80cm , Height: 2.00cm , Length: 22.80cm
Weight:   1.310kg
ISBN:  

9780896039766

ISBN 10:   0896039765
Pages:   274
Publication Date:   23 July 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of print, replaced by POD  
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Table of Contents

PNA Technology.- Chemistry.- Solid Phase Synthesis of PNA Oligomers.- Synthesis of PNA-Peptide Conjugates.- Parallel Synthesis of PNA-Peptide Conjugate Libraries.- Thermodynamics of PNA Interactions with DNA and RNA.- PNA Technology.- Measurement of PNA Binding to Double-Stranded DNA.- PNA-Mediated Immobilization of Supercoiled DNA.- PNA Openers and Their Applications.- Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes.- Diagnostics.- PNA-Mediated PCR Clamping.- PNA Clamping Techniques for the Determination of Oncogene Mutations.- PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis.- Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices.- Antisense and Antigene.- Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells.- Synthesis of Cell-Penetrating Peptide-PNA Constructs.- Antisense Inhibition of Bacterial Gene Expression and Cell Growth.- In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops.- Animal Studies.- In Vitro and In Vivo Studies on the Pharmacokinetics and Metabolism of PNA Constructs in Rodents.

Reviews

The book, Peptide Nucleic Acid, provides an excellent introduction to pNA's...provides useful information for the readers in the development of delivery systems for bioactive macromolecules. - Pharmaceutical Research

The book, Peptide Nucleic Acid, provides an excellent introduction to pNA's...provides useful information for the readers in the development of delivery systems for bioactive macromolecules. -Pharmaceutical Research

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