Overview

In Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols, a panel of world authorities present cutting-edge molecular and ultrastructure techniques for investigating the pathophysiology of oxidative stress. Described in step-by-step detail, these readily reproducible methods use the latest in immunodetection, X-ray diffraction, confocal microscopy, in situ hybridization, chemiluminescence, and cytofluorescence. Additional techniques take advantage of electron paramagnetic resonance, advanced glycation endproducts, Western blotting analysis, transcription factors, p47 phox translocation, and microsphere delivery systems. Two companion volumes, Oxidative Stress Biomarkers and Antioxidant Protocols (2002), and Free Radical and Antioxidant Protocols (1998), focus on the related biochemical and cellular biological methods.

Full Product Details

Author:   Donald Armstrong
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2002 ed.
Volume:   196
Dimensions:   Width: 15.50cm , Height: 2.20cm , Length: 23.50cm
Weight:   0.704kg
ISBN:  

9780896038516

ISBN 10:   0896038513
Pages:   356
Publication Date:   01 July 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Techniques for Histological Identification.- Cytochemical Localization of H2O2 in Biological Tissues.- Localization of Intracellular Lipid Hydroperoxides Using The Tetramethylbenzidine Reaction for Transmission Electron Microscopy.- The Immunohistochemical Localization of Glutathione Peroxidase.- 4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody.- Immunohistochemical Detection of Protein Oxidation.- Indirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolated Cells.- Techniques for Subcellular Localization.- Methods for Studying the Binding of Advanced Glycated Proteins to Receptors for Advanced Glycation Endproducts (AGE Receptors).- Measurement of Blue Fluorescence as a Protein Marker for Oxidized Membranes.- X-Ray Diffraction Analysis of Membrane Structure Changes with Oxidative Stress.- Detection of Estrogen Receptor by In Situ Hybridization.- Tumor Necrosis Factor-? Quantification and Expression by In Situ Hybridization.- Techniques for Molecular Biology.- Translocation of p47phox and Activation of NADPH Oxidase in Mononuclear Cells.- Activation of Nuclear Factor-?B (NF-?B) in Mononuclear Cells (MNC).- Assay of Intracellular Hydrogen Peroxide Generation in Activated Cytometry Individual Neutrophils by Flow.- DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs).- Analysis of Heat-Shock Transcription Factor and Element-Binding Activity.- Slot-Blot Hybridization in Studying Gene Expression During Oxygen Deprivation.- An Expression System for a Transporter of Iron and Other Metals.- Analysis of Gene Expression Following Oxidative Stress.- Measurement of Immunoglobulin G Oxidation by Western-Blot Analysis.- Thioredoxin and Redox Regulation of the Nuclear Receptor.- Analysis of Phospholipid HydroperoxideGlutathione Peroxidase mRNA.- Expression of Human Phospholipid Hydroperoxide Glutathione Peroxidase.- Techniques for In Vivo/Ex Vivo/In Vitro Applications.- Superoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence Method.- In Vivo Detection of Transition Metals and Nitrosyl-Heme Complexes Using Ex Vivo Electron Paramagnetic Resonance Spectroscopy.- Spectral-Spatial Electron Paramagnetic Resonance Imaging (EPRI) in Skin Biopsies at 9.5 GHz.- In Vivo In Situ Detection of Nitric Oxide Using Low-Frequency EPR Spectroscopy.- of NOS II Gene into Primary Cultures of Bovine and Human Endothelial Cells.- Detection of Peroxynitrite-Induced Protein and DNA Modifications.- Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine.- Liver Slice Technology as an In Vitro Model for Metabolic and Toxicity Studies.- Cytofluorescence Techniques for the Visualization of Distinct Pools of Protein Thiols at the Single Cell Level.- Preparation of Microspheres and Incorporation of Lipid Hydroperoxide for Sustained Release Studies.- Measurement of Carotenoids in the Living Primate Eye Using Resonance Raman Spectroscopy.- Interstitial Photodynamic Therapy with Moving Exposure Fiber.- Statistical Analysis.

Reviews

...this volume is unique on its focus on protocols for identification and localization of oxidants, using molecular biology and other types of applications both in-vitro and in-vivo. This book is recommended for all biomedical research libraries and researchers at all levels who are interested in oxidative stress measurement. - E-STREAMS

.,. this volume is unique on its focus on protocols for identification and localization of oxidants, using molecular biology and other types of applications both in-vitro and in-vivo. This book is recommended for all biomedical research libraries and researchers at all levels who are interested in oxidative stress measurement. - E-STREAMS

Author Information

Tab Content 6

Author Website:  

Countries Available

All regions