Overview

Enzyme kinetics, binding kinetics and pharmacological dose-response curves are currently analyzed by a few standard methods. Some of these, like Michaelis-Menten enzyme kinetics, use plausible approximations, others, like Hill equations for dose-response curves, are outdated. Calculating realistic reaction schemes requires numerical mathematical routines which usually are not covered in the curricula of life science. This textbook will give a step-by-step introduction to numerical solutions of non-linear and differential equations. It will be accompanied with a set of programs to calculate any reaction scheme on any personal computer. Typical examples from analytical biochemistry and pharmacology can be used as versatile templates. When a reaction scheme is applied for data fitting, the resulting parameters may not be unique. Correlation of parameters will be discussed and simplification strategies will be offered.

Full Product Details

Author:   Heino Prinz
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Edition:   2011 ed.
Dimensions:   Width: 15.50cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.454kg
ISBN:  

9783642208195

ISBN 10:   3642208193
Pages:   149
Publication Date:   06 August 2011
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Introduction Classical enzyme kinetics Classical dose-response curves and the Hill coefficient Classical association and dissociation kinetics and exponential fits Numerical methods Preparing the computer Installation of Octave for different platforms Installation of Linux Running the provided programs Data input and output Binding equilibria Analytical and numerical solution for equilibrium binding to one site Equilibrium binding to two or more sites. Equilibrium binding to two or more conformational states Equilibrium binding for oxygen to hemoglobin (MWC model) Equilibrium binding of more than one ligand (e.g. agonist and inhibitor) Enzyme kinetics as coupled equilibria Enzyme kinetics with competitive or noncompetitive inhibitors Dose-response curves for receptor agonists and enzyme inhibitors Binding kinetics Ligand binding kinetics of first and second order Numerical solutions of differential equations Calculation of true enzyme kinetics Dissociation by dilution and dissociation by competition ( chase ) States and sites Least squares fit to experimental data The function lsqcurvefit Comparing correct binding curves with Hill equations Comparing real binding kinetics with current multi-exponential fits Fitting experimental data Causes for errors Multi-parameter fits Correlation of parameters Selecting a model and minimizing the number of parameters Degrees of freedom Global Fits Significance of parameters in multi-parameter fits Comparing the two different approaches Model independent versus model calculations Global fit versus single fits Make ends meet: Try to analyze reported data with new methods Designing experiments for complete analysis Appendix Description of program files Sample data Comparing MATLAB and Octave code

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