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Author:   Günter Mayer ,  Marcus M. Menger
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2023
Volume:   2570
Weight:   0.566kg
ISBN:  

9781071626979

ISBN 10:   1071626973
Pages:   282
Publication Date:   28 September 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Automated ssDNA SELEX Using Histidine Tagged Target Proteins.- Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection.- Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX.- Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction.- RNA Capture-SELEX on Streptavidin Magnetic Beads.- The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE.- Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX.- Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry.- Determination of Aptamer Structure Using Circular Dichroism Spectroscopy.- Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis.- Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance.- Probing of Fluorogenic RNA Aptamers via SupramolecularFörster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog.- Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT.- Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environments.- Aptasensor for Impedimetric Detection of Lysozyme.- Biophysical Analysis of Small Molecule Binding to Viral RNA Structures.- Designing and Expressing Circular RNA Aptamers to Regulate Mammalian Cell Biology.- Enzyme-Linked OligoNucleotide Assay (ELONA).- High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics.- Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis.

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