Overview

Monoclonal Antibody Protocols provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The protocols emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell fusion technique to increase the yield of hybridomas. The book stresses antibodies produced in mice, but includes methods of producing xenogeneic hybrids that yield human, bovine, equine, and porcine monoclonal antibodies. With its detailed instructions, its comments on how to alter the various steps of a protocol in order to accommodate different materials, and its hints and tips that often make the difference between success and failure, Monoclonal Antibody Protocols provides you with a ready and indispensable source of information for preparing and using monoclonal antibodies successfully in your laboratory.

Full Product Details

Author:   William C. Davis
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1995 ed.
Volume:   45
Dimensions:   Width: 15.50cm , Height: 2.20cm , Length: 23.50cm
Weight:   0.474kg
ISBN:  

9780896033085

ISBN 10:   0896033082
Pages:   264
Publication Date:   23 May 1995
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies.- Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro.- Culture Conditions that Optimize Outgrowth of Hybridomas.- Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies.- Production of Stable Bovine-Murine Interspecies Hybrids.- Production of Monoclonal Antibodies in Swine.- Production of Monoclonal Antibodies in Horses.- Use of Electric-Field-Mediated Cell Fusion to Produce Hybridomas Secreting Monoclonal Antibodies.- Rapid Isotyping of Mouse Monoclonal Antibodies.- Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media.- Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay.- Use of Monoclonal Antibodies in Immunohistochemistry.- Use of Monoclonal Antibodies in Immunoelectron Microscopy.- Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection.- The Use of Directly and Indirectly Labeled Monoclonal Antibodies in Flow Cytometry.- Use of Monoclonal Antibodies and Flow Cytometry to Cluster and Analyze Leukocyte Differentiation Molecules.- In Vivo and In Vitro Production of Monoclonal Antibodies.- Separation of Monoclonal Antibodies from Cell-Culture Supernatants and Ascites Fluid Using Thiophilic Agarose.- Detection, Purification, and Utilization of Murine Monoclonal IgM Antibodies.- Preparation and Use of Immunoaffinity Columns with Monoclonal Antibodies Without Purification from Ascites and Tissue-Culture Medium.- Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography.- Coupling of Monoclonal Antibodies with Fluorophores.- Coupling of Monoclonal Antibodies with Biotin.- Coupling of Monoclonal Antibodies with Enzymes.- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Negative Selection.- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Positive Selection.

Reviews

...written in sufficient detail that the first-time user could successfully use the protocols...a useful purchase... - Trends in Biochemical Sciences

...written in sufficient detail that the first-time user could successfully use the protocols...a useful purchase... -Trends in Biochemical Sciences

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