Overview

The book Molecular Markers and Plant Biotechnology is a comprehensive collection of molecular marker-based techniques, presented in 40 chapters and accompanied by 578 pages of detailed information and figures. This essential resource is a must-have for those working in the field of biotechnology. In addition to the theoretical content, the book also includes practical equipment descriptions to supplement laboratory work. The inclusion of laboratory safety rules at the beginning of the text is a wise decision. The appendices provide additional support with basic calculations, principles for preparing reagents, and an abbreviations and glossary section that demonstrates the attention to detail in the texts preparation. This indispensable book is an absolute necessity for both laboratory and classroom settings in the field of biotechnology.

Full Product Details

Author:   Rukam S. Tomar ,  Manoj V. Parakhia ,  Sunil V.Patel ,  B.A. Golakiya
Publisher:   New India Publishing Agency
Imprint:   New India Publishing Agency
Dimensions:   Width: 4.20cm , Height: 22.90cm , Length: 15.20cm
Weight:   1.390kg
ISBN:  

9789380235257

ISBN 10:   9380235259
Pages:   626
Publication Date:   15 January 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1. Milestones in DNA history and biotechnology. 2. Equipments required in a molecular marker laboratory. 3. Isolation, purification and quantification of genomic DNA from plants. 4. Isolation, purification and quantification of RNA from plants. 5. Electrophoresis. 6. Molecular weight markers for gel electrophoresis. 7. Polycrylamide Gel Electrophoresis PAGE. 8. Gel electrophoresis of protein. 9. Isoenzyme. 10. Extraction of DNA fragments from Agarose Gel. 11. Why molecular markers? 12. Restriction enzyme digestion of DNA and its Agarose Gel Electrophoresis. 13. Thermocyclers PCR Machines. 14. Modifications of basic PCR technique. 15. Random amplified polymorphic DNA RAPD. 16. Restriction Fragment Length Polymorphism RFLP. 17. Amplified Fragment Length Polymorphism AFLP. 18. Simple sequence repeats Microsatellite. 19. Variable number of Tandem repeats Minisatellite. 20. Inter Simple Sequence Repeat ISSR. 21. Sequence Characterized Amplified Region SCAR. 22. Cleaved Amplified Polymorphic Sequence CAPS. 23. Single-Strand Conformation Polymorphism SSCP. 24. Retrotransposon-based markers S-SAP, IRAP, REMAP, RBIP, RGAS, SNP. 25. Silver staining. 26. Basics of marker assisted selection in crop plants. 27. Mapping populations. 28. QTL mapping. 29. Southern blotting. 30. Western blotting. 31. Northern blotting. 32. Eastern blotting. 33. DNA sequencing and designing of primers. 34. Gene transfer to plant. 35. Fluorescent in Situ Hybridization FISH. 36. Genotypic Barcoding. 37. mtDNA. 38. MicroRNA. 39. Graphical approach to calculate molecular weights of DNA/protein fragments. 40. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.

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Author Information

Rukam S. Tomar: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat. Professor Manoj V. Parakhia:Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat, Professor Sunil V.Patel: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat. Professor. B.A. Golakiya: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat.

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