Overview

This volume provides readers with a detailed collection of in vitro and in vivo protocols developed to study inflammation and the interactions between host and micro-organisms. The chapters in this book cover a range of biological systems and methodological approaches such as direct inoculation of pathogenic yeasts into mouse lung by trans-tracheal instillation; murine models of experimental periodontal disease; quantitative in vitro and in vivo assays to assess the efferocytic efficiency of macrophages; murine models of alphavirus infection to study the immunopathogenesis of pain; and in vivo preclinical platforms to study coronavirus infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Models in Inflammation and Immunopharmacology is a practical and conceptual resource for researchers studying inflammation and host-microorganism interactions. This book is also a valuable reference for educators, clinicians, and policymakers who want to understand the foundations of experimental design in biomedicine.  

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9781071650189

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