Overview

This volume provides a comprehensive guide of cutting-edge technologies applied in the discovery of metabolic gene clusters across different domains of life. Chapter guide readers through cluster identification, characterization, evolutionary analyses, synthetic assembly, pathway re-constitution, cluster regulation and activation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Metabolic Gene Clusters: Methods and Protocols aims to spur new discoveries in the field of metabolic gene clusters and bring different areas of research closer together.

Full Product Details

Author:   Hans-Wilhelm Nützmann ,  Ramesha Thimmappa
Publisher:   Springer-Verlag New York Inc.
Imprint:   Humana
ISBN:  

9781071653838

ISBN 10:   1071653830
Pages:   176
Publication Date:   31 July 2026
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Forthcoming
Availability:   Not yet available  
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Table of Contents

Generalized Gene Cluster Detection Using CLOCI.- In Silico Identification and Comparative Synteny of Biosynthetic Gene Clusters in Plants.- Mining Metabolic Diversities Across Genomic Neighborhoods in Plants.- Identification of Stress-induced Biosynthetic Gene Clusters in Plants.- Investigating Intraspecific Diversity of Metabolic Gene Clusters with MLPA and Synthetic Probes.- Synteny Analysis and Visualization of Gene Clusters.- Automating Plasmid Construction for High-throughput Heterologous Expression of Fungal Gene Clusters.- Molecular Lego in the Green Factory: Engineering Plant Metabolism with Golden Gate Assembly.- Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.- Characterization of Bioactive Saponins from Sea Cucumbers.- Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.- Cut&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.- Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.- Mapping the 3D Chromosome Organisation of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

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