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Laboratory Techniques in Biochemistry and Molecular Biology

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Author:   A. Kuksis (Banting and Best Department of Medical Research, University of Toronto, Canada)
Publisher:   Elsevier Science & Technology
Imprint:   Elsevier Science Ltd
Volume:   v.30
Dimensions:   Width: 15.90cm , Height: 4.10cm , Length: 24.10cm
Weight:   1.130kg
ISBN:  

9780444513045

ISBN 10:   0444513043
Pages:   1000
Publication Date:   12 September 2003
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Chapter 1. General introduction. 1.1 Introduction. 1.2 Natural occurrence. 1.3 Biological significance. 1.4 Nomenclature. 1.5 Scope of the book. 1.6 References. Chapter 2. Phosphatidylinositols. 2.1 Isolation. 2.2 Identification. 2.3 Quantification. 2.4 References. Chapter 3. Phosphatidylinositol phosphates. 3.1 Isolation. 3.2 Determination of structure. 3.3 Resolution of molecular species. 3.4 Quantification. 3.5 References. Chapter 4. Inositol phosphates. 4.1 Introduction. 4.2 Isolation. 4.3 Resolution of chemical subclasses. 4.4 Resolution of positional isomers. 4.5 Determination of chemical structure. 4.6 Determination of stereochemical structure. 4.7 Quantification. 4.8 References. Chapter 5. Glycosylphosphatidylinositols. 5.1 Introduction. 5.2 National occurrence and isolation. 5.3 Determination of chemical structure. 5.4 Determination of molecular species. 5.5 Quantification. 5.6 Related structures. 5.7 References. Chapter 6. Biosynthesis of inositol phospholipids. 6.1 Introduction. 6.2 Phosphatidylinositols. 6.3. Phosphatidylinositol phosphates. 6.4 Glycosyl phosphatidylinositols. 6.5. References. Chapter 7. Phosphatidylinositol and inositol phosphate kinases. 7.1 Introduction. 7.2 Phosphatidylinositol kinases. 7.3 Phosphatidylinositol monophosphate kinases. 7.4 Phosphatidylinositol bisphosphate kinases. 7.5 Inositol monophosphate kinases. 7.6 Inositol bisophosphate kinases. 7.7 Inositol trisphosphate kinases. 7.8 Inositol tetrakisphosphate kinases. 7.9 Inositol pentakisphosphate kinases. 7.10 Inositol hexakisphosphate kinases. 7.11 References. Chapter 8. Phosphatidylinositol phosphate and inositol phosphate phosphatases. 8.1 Introduction. 8.2 PtdIns phosphate phosphatases. 8.3 Inositol phosphate phosphatases. 8.4 Other phosphatases and phytases. 8.5 References. Chapter 9. Phosphatidylinositol phospholipases. 9.1 Introduction. 9.2 PtdIns-nonspecific phospholipases. 9.3 PtdIns-specific phospholipases. 9.4 PtdIns(4,5)P2 activated phospholipases. 9.5 PtdIns(3,4,5)P3 activated phospholipases. 9.6 References. Chapter 10. Preparation of standards. 10.1 Introduction. 10.2 Phosphatidylinositol phosphates. 10.3 Inositol phosphates. 10.4 Inositol pyrosphosphates. 10.5 Structural analogues. 10.6 References. Chapter 11. InsPs and PtdInsPs as signaling molecules. 11.1 Introduction. 11.2 InsPs as cellular signals. 11.3 PtdInsPs as cellular signals. 11.4 References.

Reviews

In this excellent new book, Kuksis shows how our knowledge of the remarkable activities of these compounds has only been possible because of the continuous development of novel analytical methodologies...In short, it is hard to conceive of a more comprehensive and thorough guide to the subject of analysis and biochemistry of phosphatidylinositol and its metabolites. It is quite remarkable that this substantial book has been produced by single author, especially as this leads to a uniformity of treatment in the various chapters impossible to achieve in multi-author volumes. Every biochemistry and lipid library should have a copy. --William W. Christie, MRS Lipid Analysis Unit, Scottish Crop research Institute, Invergowrie, Dundee, Scotland for LIPID TECHNOLOGY NEWSLETTER, December 2003

Every biochemistry and lipid library should have a copy. --LIPID TECHNOLOGY NEWSLETTER, December 2003

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