Overview

The Immunology Methods Manual is a four-volume set comprising a comprehensive sourcebook of immunological methods and techniques. Unique in its project-oriented, rather than protocol-oriented approach, the emphasis is placed on describing the environment in which each method was developed and is used. The manual contains both advanced methods and descriptions and basic protocols. It is essential not only for those who study the immune system of man or mouse, but also those applying methods to other species. This new compilation of methods answers the needs of immunologists, molecular, developmental, and cell biologists, students and advanced researchers, and workers in clinical and diagnostic laboratories. Protocols are provided to guide day-to-day laboratory work as well as the development of long-term strategies. Vital contact information also encourages useful dialogue and interaction between authors and method-users. Led by Ivan Lefkovits and five internationally recognized advisory editors, more than 35 section editors and 350 authors have contributed to compile this four-volume practical guide to methods that work.

Full Product Details

Author:   Ivan Lefkovits (Basel Institute for Immunology, Basel, Switzerland)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Dimensions:   Width: 14.40cm , Height: 1.00cm , Length: 12.50cm
Weight:   0.100kg
ISBN:  

9780124427150

ISBN 10:   0124427154
Publication Date:   13 December 1996
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   CD-ROM
Publisher's Status:   Unknown
Availability:   Out of stock  

Table of Contents

Expression of Recombinant Proteins. Engineered Molecules. Gene Targeting. Monitoring V(D)J Rearrangement. The Art of Polymerase Chain Reaction (PCR). Transcriptional Gene Regulation in B and T Cells. Mrna Expression. Antibody and Immune Complexes. MHC Ligands and Peptide Binding. Screening of Cells of the B Lineage. Methods to Study B Lymphopoiesis in Mouse and Man. Modulation of the Humoral Immune Response and Its Measurement. Signaling Pathways and Lymphocyte Cell Repertoire. Immunohistological Tools. Lymphocytes as Single Cells. Lymphoid Lines as Models for Physiological Cell Death. Leukocyte Migration and Adhesion. Mast Cells. Dendritic Cells of Mice and Humans. Isolation of Lymphocytes from Non-Lymphocytes from Non-Lymphoid Tissues. Gnorobiological Models. Interaction of Peptidoglycanpolysaccharides from the Human Intestinal Flora Bacteria and the Immune System. Rheumatology in Relation to Immune Response and Microbes. Experimental Models of Organ-Specific Autoimmunity (Nervous System). Experimental Models of Autoimmune Methods. Immunodiagnosis of B-Cell Malignancies. VSV, LCMV and Their Targets. HIV and the Immune Response. In Vivo Techniques of Experimental Immunology in Sheep. In Vitro Techniques of Experimental Immunology in Sheep. Experimental Immunology in Chickens. Cold Blooded Vertebrates.

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