Overview

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences. The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing volume on nucleic acid extraction strategies. Due to the complexities within prokaryotic and eukaryotic cells, purification of the nucleic acids often forms a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens. Bringing together contributions from leading researchers, the handbook presents a comprehensive catalog of nucleic acid isolation methods. It includes dedicated sections on strategies for viruses, bacteria, fungi, parasites, insects, mammals, and plants, as well as for ancient samples, with an additional emphasis on sample preparation methods for direct molecular applications. Each chapter in this handbook: * Explores the biological background important to understanding specific organisms and specimens * Reviews principles and current techniques for efficient isolation * Discusses challenges and future trends relating to improved recovery of nucleic acids Besides providing an updated, reliable reference for anyone with an interest in molecular biology, this book offers a practical guide for clinical, forensic, and research scientists involved in molecular analysis of biological specimens. It also constitutes a convenient resource for students in other areas of biological sciences, and an indispensable roadmap for both new and experienced researchers wishing to acquire or sharpen their skills in nucleic acid preparation.

Full Product Details

Author:   Dongyou Liu
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
ISBN:  

9781420070972

ISBN 10:   1420070975
Pages:   584
Publication Date:   14 January 2009
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Electronic book text
Publisher's Status:   Active
Availability:   Available To Order  
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Table of Contents

Nucleic Acids: Structures, Functions, and Applications, Valentin V. Vlassov, Dmitrii V. Pyshnyi, and Pavel E. Vorobjev Purification of Nucleic Acids from Viruses Isolation of Viral DNA from Cultures, Larry A. Hanson Isolation of Viral RNA from Cultures, Ling Zhao and Zhen F. Fu Preparation of Viral Samples for Direct Molecular Applications, Grant S. Hansman Purification of Nucleic Acids from Bacteria Chapter 5 Isolation of Bacterial DNA from Cultures, Dongyou Liu Isolation of Bacterial RNA from Cultures, Som Subhra Chatterjee and Trinad Chakraborty Isolation of Plasmids, Armelle Bigot and Alain Charbit Preparation of Bacterial Samples for Direct Molecular Applications, Knut Rudi, Robert C. Wilson, Kjetill S. Jakobsen, and Dongyou Liu Purification of Nucleic Acids from Fungi Isolation of Nucleic Acids from Yeasts, Lakshman P. Samaranayake, Nipuna B. Parahitiyawa, and C. Jayampath Seneviratne Isolation of Nucleic Acids from Filamentous Fungi, Margaret E. Katz and Brian F. Cheetham Preparation of Fungal Specimens for Direct Molecular Applications, Francoise Irlinger, Nicolas Berthet, Tatiana Vallaeys, Valerie Vasseur, Renaud Ioos, Marc Buee, and Jerome Mounier Purification of Nucleic Acids from Parasites and Insects olation of Nucleic Acids from Protozoa, Vitaliano A. Cama and Lihua Xiao Isolation of Nucleic Acids from Helminthes, Munehiro Okamoto and Akira Ito Isolation of Nucleic Acids from Insects, Kun Yan Zhu Preparation of Parasitic Specimens for Direct Molecular Applications, Huw Vaughan Smith and Rosely Angela Bergamin Nichols Purification of Nucleic Acids from Mammals Isolation of Nucleic Acids from Body Fluids, Karen Page and Jacqueline Amanda Shaw Isolation of Nucleic Acids from Soft Tissues, Sergey Kovalenko Isolation of Nucleic Acids from Hard Tissues. Judith Weidenhofer and Jennifer A. Byrne Isolation of DNA from Ancient Samples, Paula F. Campos, Eske Willerslev, and M. Thomas P. Gilbert Preparation of Forensic Samples for Direct Molecular Applications, Erin K. Hanson and Jack Ballantyne Purification of Nucleic Acids from Plants Isolation of Nucleic Acids from Plants, Song Weining and Dongyou Liu Isolation of Megabase-Sized DNA Fragments from Plants, Meiping Zhang, Yaning Li, and Hong-Bin Zhang Purification of Nucleic Acids from Miscellaneous Sources Purification of Nucleic Acids from Gels, Chunfang Zhang and Theo Papakonstantinou Index

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