Overview

DNA Sequencing Protocols, Second Edition, empowers researchers with the tools and knowledge required to become a sequencing expert. It covers a wide range of DNA sequencing technologies in common usage in molecular biology laboratories. It acts as a laboratory guide to DNA sequencing and it helps students and researchers to understand the instrumentation (ABI, ALF & LiCor) and chemistries involved. The reader is also given Internet addresses for Genome sequencing sites and step by step guides to the use of sequence analysis software and databases i. e. for BLAST and NIX searches. The book also reviews some of the instrumentation available for automation of DNA sequencing processes which is in more widespread use in many research groups. Some of the protocols covered in detail include, M13 cloning and sequencing; Primer design; PCR product sequencing; Cycle sequencing; Solid phase sequencing; Shotgun sequencing; Fluorescent sequencing for 310, 373, 377, ALF, LiCor; Heterozygote mutation detection; Automation, and; Sequence databases and the Internet. The book will also be useful to clinicians with research interests to update them on current DNA sequencing methods, applications and future prospects. DNA sequencing has become an essential tool for molecular diagnostics with the completion of many genome projects.

Full Product Details

Author:   Barry W. Hicks
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   183
Dimensions:   Width: 15.50cm , Height: 2.30cm , Length: 23.50cm
Weight:   0.774kg
ISBN:  

9780896039056

ISBN 10:   0896039056
Pages:   394
Publication Date:   20 June 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of print, replaced by POD  
We will order this item for you from a manufatured on demand supplier.

Table of Contents

Manipulation of Green Fluorescent Protein Structure at the Genetic Level.- Amplification of Representative cDNA Samples from Microscopic Amounts of Invertebrate Tissue to Search for New Genes.- Use of cobA and cysGA as Red Fluorescent Indicators.- Circular Permutation of the Green Fluorescent Protein.- Evolutionary Design of Generic Green Fluorescent Protein Biosensors.- Random Insertion of Green Fluorescent Protein into the Regulatory Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase.- Circular mRNA Encoding for Monomeric and Polymeric Green Fluorescent Protein.- Detection and Imaging of Green Fluorescent Protein.- Fluorescence Lifetime Imaging (FLlM) of Green Fluorescent Fusion Proteins in Living Cells.- 8 Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein.- Bioluminescence Resonance Energy Transfer Assays for Protein-Protein Interactions in Living Cells.- Whole-Body Fluorescence Imaging with Green Fluorescence Protein.- Green Fluorescent Protein to Monitor Protein Distribution and Trafficking.- Drug-Induced Translocation of Nucleolar Proteins Fused to Green Fluorescent Protein.- Light-Induced Nuclear Targeting of PhytochromeB-sGreen Fluorescent Protein in Plants.- Mechanisms of Protein Trafficking.- Analysis of Nucleocytoplasmic Transport Using Green Fluorescent Protein.- Green Fluorescent Protein in Transgenic Organisms.- Transgenic Bovine Embryo Selection Using Green Fluorescent Protein.- Development of Glycosylphosphatidylinositol-Anchored Enhanced Green Fluorescent Protein.- Transgenic Zebrafish Expressing Green Fluorescent Protein.- Transgenic Insects Expressing Green Fluorescent Protein-Silk Fibroin Light Chain Fusion Protein in Transgenic Silkworms.- Green Fluorescent Protein inTransgenic Plants.- GreenFluorescent Protein Biosensors.- Green Fluorescent Protein Calcium Biosensors.- Green Fluorescent Protein Fluobody Immunosensors.- 22 Green Fluorescent Protein-Based Protein Kinase Biosensor Substrates.- Green Fluorescent Protein Urea Sensors.- Viral Applications of Green Fluorescent Protein.- Using Green Fluorescent Protein to Monitor Measles Virus Cell-to-Cell Spread by Time-Lapse Confocal Microscopy.- Tracking and Selection of Retrovirally Transduced Murine Bone Marrow Cells Using Green Fluorescent Protein.- Green Fluorescent Protein as a Reporter of Adenovirus-Mediated Gene Transfer and Expression in the Hypothalamic-Neurohypophyseal System.- Enhancement of Green Fluorescent Protein Expression in Adeno-Associated Virus with the Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Element.- Construction of Infectious Simian Varicella Virus Expressing Green Fluorescent Protein.- Green Fluorescent Protein in Retroviral Vector Constructs as Marker and Reporter of Gene Expression for Cell and Gene Therapy Applications.

Reviews

The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value. -Doody's Health Sciences Book Review Journal ...this book truly is a collection of Applications and protocols and such is the degree of detail in the methods described that it should perhaps have been sub-titled A Laboratory Manual. -The Analyst

A particularly helpful adjunct is provided by a CD-ROM included with the book. This disc contains color images for many of the protocols and is especially useful for visualization. The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value. -Doody's Health Sciences Book Review Journal ...this book truly is a collection of Applications and protocols and such is the degree of detail in the methods described that it should perhaps have been sub-titled A Laboratory Manual. -The Analyst

"""The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value."" -Doody's Health Sciences Book Review Journal ""...this book truly is a collection of ""Applications and protocols"" and such is the degree of detail in the methods described that it should perhaps have been sub-titled ""A Laboratory Manual."" -The Analyst"

A particularly helpful adjunct is provided by a CD-ROM included with the book. This disc contains color images for many of the protocols and is especially useful for visualization. The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value. - Doody';s Health Sciences Book Review Journal ...this book truly is a collection of Applications and protocols and such is the degree of detail in the methods described that it should perhaps have been sub-titled A Laboratory Manual. - The Analyst

The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value. -Doody's Health Sciences Book Review Journal ...this book truly is a collection of Applications and protocols and such is the degree of detail in the methods described that it should perhaps have been sub-titled A Laboratory Manual. -The Analyst

The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value. -Doody's Health Sciences Book Review Journal ...this book truly is a collection of Applications and protocols and such is the degree of detail in the methods described that it should perhaps have been sub-titled A Laboratory Manual. -The Analyst

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