Overview

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

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9781588290502

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this volume will be worth returning to for years to come the spirit of this volume is not that it will get you through, but that it will make you comfortable with the technology and put you in touch with the art form a valuable and stimulating source of essential technical information for all experimental drosophilists'. Nature Genetics

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