DNA Cloning 1: A Practical Approach: Core Techniques
Author:   Glover ,  Hames
Publisher:   Oxford University Press
Edition:   2nd Revised edition
Volume:   148
ISBN:  

9780199634767


Pages:   288
Publication Date:   11 May 1995
Format:   Paperback
Availability:   In Print   Availability explained
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DNA Cloning 1: A Practical Approach: Core Techniques


Overview

This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date account of each topic by established researchers. These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

Full Product Details

Author:   Glover ,  Hames
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   2nd Revised edition
Volume:   148
Dimensions:   Width: 15.70cm , Height: 1.60cm , Length: 23.50cm
Weight:   0.499kg
ISBN:  

9780199634767


ISBN 10:   0199634769
Pages:   288
Publication Date:   11 May 1995
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

1. Techniques for transformation of E.coli ; 2. Lambda replacement vectors in the construction of genomic DNA libraries ; 3. Procedures for cDNA cloning ; 4. Making probes ; 5. Using cloned DNAs to analyse gene organization and expression ; 6. Choices of single-stranded vectors and their application for in vitro mutagenesis ; 7. PCR and its applications ; 8. DNA sequencing

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