Overview

This book provides descriptions of experimental methods in research on the cytoskeleton and its relationships to signaling and cell regulation. Thus, it bridges two active and fertile areas of research. The focus is directed particularly towards methods which take advantage of recent advances in molecular biology, microscopy and immunological assays. A second emphasis is on methods for understanding dynamic changes in cells. A third emphasis is on the formation and turnover of macromolecular and supramolecular complexes, which are so important in driving cell regulation and the behaviour of cytoskeletal elements. A combination of practical advice and detailed protocols should make this book valuable for both novice and experienced workers in these burgeoning fields.

Full Product Details

Author:   Kermit Carraway (Department of Cell Biology, University of Miami) ,  Coralie Carraway (Department of Biochemistry, University of Miami)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   New edition
Volume:   221
Dimensions:   Width: 15.60cm , Height: 1.80cm , Length: 23.40cm
Weight:   0.549kg
ISBN:  

9780199637812

ISBN 10:   0199637814
Pages:   308
Publication Date:   16 December 1999
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Preface 1: Carraway: Introduction: The cytoskeleton in the transduction of signal and regulation of cellular function 2: Ampe and Vandekerckhove: Assaying binding and covalent modifications of cytoskeletal proteins 3: Karpova and Cooper: Methods to study actin assembly and dynamics in yeast 4: Carraway and Carraway: Signaling complexes: Association of signaling proteins with the cytoskeleton 5: Ridley and Flinn: Ras-related GTPases and the cytoskeleton 6: Fishkind et al: Applications for analysis of contractile functions in dividing and migrating tissue culture cells 7: Zeile et al: Video microscopy: Protocols for examining the actin-based motility of Listeria, Shigella, Vaccinia, and Lanthanum-induced endosomes. 8: Downing: Structural studies on tubulin and microtubules 9: Inada et al: Regulation of Intermediate Filament Dynamics 10: Bermano and Hesketh: The study of mRNA-cytoskeleton interactions and mRNA sorting in mammalian cells 11: Goldmann et al: Cell shape control and mechanical signaling through the cytoskeleton Appendix Index

Reviews

In this volume, experimental techniques are presented as a method to study the cytoskeleton from the perspective of signaling and cell regulation. The book is written for the scientist doing bench research on the cytoskeleton. The chapter contributors are knowledgeable scientists in their fields. Many methods for purifying, co-purifying, labeling, and/or perturbing the various components of the cytoskeleton are presented. -- Doody's<br>

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