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Author:   Stephen M. King (Professor, Department of Molecular Biology and Biophysics Director, Electron Microscopy Facility, University of Connecticut Health Center) ,  Gregory J Pazour
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Edition:   91st edition
Volume:   91
Dimensions:   Width: 18.40cm , Height: 1.90cm , Length: 26.00cm
Weight:   0.740kg
ISBN:  

9780123749734

ISBN 10:   0123749735
Pages:   344
Publication Date:   21 December 2009
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1. RNAi approaches to axonemal motor function in trypanosomes (Hill) 2. Homologous recombination to knock out dynein genes in Tetrahymena (Gaertig) 3. RNAi in Chlamydomonas (Cerutti) 4. Planarians as a model system for analysis of ciliary assembly/motility (Rompolas) 5. Role of nucleotides in dynein function (Shingyoji) 6. Protein modification to probe dynein interactions + purification and identification of crosslinked dynein products (Sakato) 7. Analysis of redox-sensitive dynein components (Wakabayashi) 8. Purification of dyneins from Chlamydomonas and analysis of dynein-dynein linkers (Kamiya/King) 9. Kinase/phosphatase-mediated control of dynein function (Sale) 10. Central pair MT complex and associated kinesins (Mitchell) 11. Calcium regulation of axonemal function In vitro motility assays (Smith) 12. Identification and analysis of dynein regulatory complex components (Porter) 13. Isolation and analysis of radial spoke proteins (Yang) 14. Purification of dyneins from other model organisms: Ciona, sea urchin, fish (Inaba) 15. Rescue of mutant phenotypes by protein electroporation (Kamiya Lab) 16. Tubulin/Dynein interactions (Raff) 17. Measurement of beat frequency and swimming velocity Cell model reactivation in vitro assays of dynein function (Kamiya) 18. Live imaging of ependymal cilia (Lechtreck/Witman) 19. Immunogold labeling of axonemal components in situ + flat embedding (Geimer) 20. CryoEM approaches to axonemal organization (Nicastro) 21. CryoEM of dynein-microtubule complexes (Oda/Kikkawa) 22. Bioinformatic approaches to dynein HC classification (Yagi) 23. Bioinformatics of non-motor dynein components (Asai) 24. Biophysical approaches to dynein mechanism (Sutoh) 25. Chlamydomonas flagellar beat analysis (Foster) 26. Biophysical measurements of motor function (step size/force production) SAXS of axonemes (Toba/Oiwa) Cryo-Electron Microscope Tomography to Study Axonemal Organization Daniela Nicastro 2. Electron Microscopic Imaging and Analysis of Isolated Dynein Particles Anthony J. Roberts and Stan A. Burgess 3. Immunogold Labeling of Flagellar Components In Situ Stefan Geimer 4. Scanning Electron Microscopy to Examine Cells and Organs Jovenal T. SanAgustin, John A. Follit, Gregory Hendricks, and Gregory J. Pazour 5. X-ray Fiber Diffraction Studies on Flagellar Axonemes Kazuhiro Oiwa, Shinji Kamimura, and Hiroyuki Iwamoto 6. Markers for Neuronal Cilia Jacqueline S. Domire and Kirk Mykytyn 7. Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells Heymut Omran and Niki T. Loges 8. Immunoprecipitation to Examine Protein Complexes Gregory J. Pazour 9. Tandem Affinity Purification of Ciliopathy-Associated Protein Complexes Karsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner, Marius Ueffing, and Ronald Roepman 10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products Miho Sakato 11. Analysis of the Ciliary/Flagellar Beating of Chlamydomonas Kenneth W. Foster 12. Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii Ritsu Kamiya 13. High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain Karl-Ferdinand Lechtreck, Michael J. Sanderson, and George B. Witman 14. Observation of Nodal Cilia Movement and Measurement of Nodal Flow Yasushi Okada and Nobutaka Hirokawa 15. Modification of Mouse Nodal Flow by Applying Artificial Flow Shigenori Nonaka 16. Measuring Cilium-Induced Ca2þ Increases in Cultured Renal Epithelia Helle A. Praetorius

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Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

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