Overview

Leading cell biologists and immunologists present their most useful and innovative techniques for studying the molecular and cellular basis of cell migration in the inflammatory process. The authors provide a series of focused, cutting-edge techniques proceeding from the in vitro analysis of cell migration and the molecular mechanisms underlying this process, to methodologies for the analysis of cell migration in vivo. Experimental approaches useful in establishing the role of cell migration in the pathogenesis of both acute and chronic inflammatory diseases are emphasized. Methods for the analysis of rapid leukocyte adhesion under flow conditions in vitro are described, which may prove especially fruitful for scientists exploring the molecular mechanisms underlying vascular recognition and leukocyte endothelium interaction. Each fully tested protocol includes an introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and how to avoid known pitfalls.

Full Product Details

Author:   Daniele D’Ambrosio ,  Francesco Sinigaglia
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2003 ed.
Volume:   239
Dimensions:   Width: 15.50cm , Height: 1.70cm , Length: 23.50cm
Weight:   1.290kg
ISBN:  

9781588291028

ISBN 10:   1588291022
Pages:   277
Publication Date:   29 August 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Chemotaxis and Interaction with Vascular or Lymphatic Endothelium.- Analysis of Integrin-Dependent Rapid Adhesion Under Laminar-Flow Conditions.- Posttranslational Processing of Chemokines.- Chemotactic Profiling of Lymphocyte Subpopulations.- Measurement of the Levels of Polymerized Actin (F-Actin) in Chemokine-Stimulated Lymphocytes and GFP-Coupled cDNA Transfected Lymphoid Cells by Flow Cytometry.- Evaluation of Rho Family Small G-Protein Activity Induced by Integrin Ligation on Human Leukocytes.- Reconstructing Leukocyte Migration in 3D Extracellular Matrix by Time-Lapse Videomicroscopy and Computer-Assisted Tracking.- Analyzing Chemotaxis Using Dictyostelium discoideum as a Model System.- Conditional Transgenic Models to Study Chemokine Biology.- Intravital Microscopy as a Tool for Studying Recruitment and Chemotaxis.- Tracking Antigen-Specific Lymphocytes In Vivo.- Analysis of Homing-Receptor Expression on Infiltrating Leukocytes in Disease States.- Interaction of Viral Chemokine Inhibitors with Chemokines.- Discovery of Small-Molecule Antagonists of Chemokine Receptors.- Visualization and Analysis of Adhesive Events in Brain Microvessels by Using Intravital Microscopy.- Animal Models to Study Chemokine Receptor Function In Vivo.- Assessing the Role of Multiple Phosphoinositide 3-Kinases in Chemokine Signaling.- In Vitro and In Vivo Models to Study Chemokine Regulation of Angiogenesis.- Real-Time In Vitro Assay for Studying Chemoattractant-Triggered Leukocyte Transendothelial Migration Under Physiological Flow Conditions.- Generation of Monoclonal Antibodies Against Chemokine Receptors.- Detection of High-Affinity ?4-Integrin Upon Leukocyte Stimulation by Chemoattractants or Chemokines.

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