Overview

This collection of cutting edge techniques for the study of the eukaryotic cell cycle and its key regulatory molecules includes overviews of cell cycle regulatory mechanisms in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on well-defined cell cycle regulators - and those more recently defined - in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The book offers all cell researchers indispensable techniques essential to understanding how normal cells divide and how this is altered in disease.

Full Product Details

Author:   Tim Humphrey ,  Gavin Brooks
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2005 ed.
Volume:   296
Dimensions:   Width: 15.50cm , Height: 2.30cm , Length: 23.50cm
Weight:   1.670kg
ISBN:  

9781588291448

ISBN 10:   1588291448
Pages:   404
Publication Date:   01 December 2004
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Overviews Of Cell Cycle Control In Different Organisms.- Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts.- The Plant Cell Cycle.- The C. elegans Cell Cycle.- Developmental Control of Growth and Cell Cycle Progression in Drosophila.- The Xenopus Cell Cycle.- The Mammalian Cell Cycle.- Cell Cycle Study Methods And Techniques.- Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle.- Mapping Origins of DNA Replication in Eukaryotes.- In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast.- Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short Hairpin RNAs.- The Cell Cycle and Virus Infection.- Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies.- Overviews Of Cell Cycle Control In Different Organisms.- E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression.- Forkhead (FOX) Transcription Factors and the Cell Cycle.- Measurement of Geminin Activity in Xenopus Egg Extracts.- CDK-Activating Kinases.- Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors.- Measurement of Wee Kinase Activity.- CDC25 Dual-Specificity Protein Phosphatases.- Assaying Cell Cycle Checkpoints.- Polo-Like Kinase-1.- The Ipl1/Aurora Kinase Family.- Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs.

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