Overview

Expert laboratory researchers describe in a standard format all the diverse laboratory methods needed to perform state-of-the-art tissue engineering. Topics range from the synthesis, processing, and characterization of specific biomaterials, through the successful use of scaffolds in the engineering of tissues, to techniques useful in evaluating the biological quality of scaffold-engineered tissues. Topics of special interest include the incorporation of biological molecules into scaffold biomaterials and the use of a wide range of methods and techniques to generate a comprehensive description of cell polymer construct quality.

Full Product Details

Author:   Anthony P. Hollander ,  Paul V. Hatton
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   238
Dimensions:   Width: 15.50cm , Height: 1.70cm , Length: 23.50cm
Weight:   1.260kg
ISBN:  

9780896039674

ISBN 10:   0896039676
Pages:   257
Publication Date:   17 November 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Processing of Resorbable Poly-?-Hydroxy Acids for Use as Tissue-Engineering Scaffolds.- Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound Healing.- Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineering.- Synthesis and Characterization of Chitosan Scaffolds for Cartilage-Tissue Engineering.- Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2.- Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tissue-Engineering Applications.- Alginates in Tissue Engineering.- Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering.- Modification of Materials With Bioactive Peptides.- Isolation and Osteogenic Differentiation of Bone-Marrow Progenitor Cells for Application in Tissue Engineering.- Cell Seeding of Polymer Scaffolds.- Chondrocyte Isolation, Expansion, and Culture on Polymer Scaffolds.- Bioreactor Culture Techniques for Cartilage-Tissue Engineering.- Microscopic Methods for the Analysis of Engineered Tissues.- Transmission Electron Microscopy of Tissue-Polymer Constructs.- Application of Microscopic Methods for the Detection of Cell Attachment to Polymers.- Biochemical Methods for the Analysis of Tissue-Engineered Cartilage.- Real-Time Quantitative RT-PCR Assays.- Mechanical Testing of Cell-Material Constructs: A Review.

Reviews

From the reviews: This small, 280-page volume is useful to people who are interested in using various laboratory techniques to conduct tissue engineering research. ! Tips on troubleshooting and avoiding pitfalls which appear at the end of nearly all chapters will be welcomed by users of the book. The goal of this book is to 'serve as a basic laboratory manual allowing tissue engineering scientists not only to access a wide range of techniques in one place, but also to have them described using a standard format'. (Annales de Chimie Science des Materiaux, Vol. 29 (4), 2004)

From the reviews: <p> This small, 280-page volume is useful to people who are interested in using various laboratory techniques to conduct tissue engineering research. a ] Tips on troubleshooting and avoiding pitfalls which appear at the end of nearly all chapters will be welcomed by users of the book. The goal of this book is to a ~serve as a basic laboratory manual allowing tissue engineering scientists not only to access a wide range of techniques in one place, but also to have them described using a standard formata (TM). (Annales de Chimie Science des Materiaux, Vol. 29 (4), 2004)

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