Overview

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

Full Product Details

Author:   Cheryl D. Helgason
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   3rd Revised edition
Volume:   v. 290
Dimensions:   Width: 15.20cm , Height: 2.20cm , Length: 22.90cm
Weight:   0.001kg
ISBN:  

9781588295453

ISBN 10:   1588295451
Pages:   384
Publication Date:   15 October 2004
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Mixed media product
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line Cheryl D. Helgason Detection of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Eradication of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Authentication of Scientific Human Cell Lines: Easy-to-Use DNA Fingerprinting Wilhelm G. Dirks and Hans G. Drexler Cytogenetic Analysis of Cell Lines Roderick A. F. MacLeod and Hans G. Drexler Human and Mouse Hematopoietic Colony-Forming Cell Assays Cindy L. Miller and Becky Lai Isolation and Culture of Murine Macrophages John Q. Davies and Siamon Gordon Isolation and Culture of Human Macrophages John Q. Davies and Siamon Gordon Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture Tomoo Ueno, Cunlan Liu, Takeshi Nitta, and Yousuke Takahama In Vitro Generation of Lymphocytes From Embryonic Stem Cells Renee F. de Pooter, Sarah K. Cho, and Juan Carlos Zuniga-Pflucker Hematopoietic Development of Human Embryonic Stem Cells in Culture Xinghui Tian and Dan S. Kaufman Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros Region Robert A. J. Oostendorp, Kirsty Harvey, and Elaine A. Dzierzak Culture of Human and Mouse Mesenchymal Cells Emer Clarke Isolation, Purification, and Cultivation of Murine and Human Keratinocytes Frizell L. Vaughan and Ludmila I. Bernstam Isolation and Culture of Primary Human Hepatocytes Edward L. LeCluyse, Eliane Alexandre, Geraldine A. Hamilton, Catherine Viollon-Abadie, D. James Coon, Summer Jolley, and Lysiane Richert Primary Kidney Proximal Tubule Cells Mary Taub Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity John Stingl, Joanne T. Emerman, and Connie J. Eaves Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue Sharon A. Louis and Brent A. Reynolds Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells Gabi Shefer and Zipora Yablonka-Reuveni Adult Ventricular Cardiomyocytes: Isolation and Culture Klaus-Dieter Schluter and Daniela Schreiber Isolation and Culture of Primary Endothelial Cells Bruno Larrivee and Aly Karsan Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions Susan L. Cuvelier and Kamala D. Patel Isolation and Characterization of Side Population Cells Margaret A. Goodell, Shannon McKinney-Freeman, and Fernando D. Camargo Scalable Production of Embryonic Stem Cell-Derived Cells Stephen M. Dang and Peter W. Zandstra Index

Reviews

From Reviews of the Second Edition... a highly prized collection that no cell culture laboratory should be without, so much so that it is likely to be borrowed' and never returned! - Retinoids & Lipid Soluble Vitamins in Clinical Practice any laboratory involved in cell culture will find this book of use - Microbiology Today would be most useful to have in the laboratory itself and a valuable reference resource in a library. Four Stars - Doody's Health Sciences Book Review Journal an excellent starting point for those who are interested in learning the basics of cell culture and the types of manipulations that make them useful. - Journal of Chemical Neuroanatomy

Aus den Rezensionen zur 3. Auflage: ! eine Sammlung von 24 Beitragen, die ... von ausgewiesenen Autoren verfasst wurden ... Ein ausfuhrliches einleitendes Kapitel ! beschaftigt sich mit grundsatzlichen Themen. Der Leser erhalt viele wichtige Informationen uber das korrekte Auftauen von Zellen, Memurnwechsel, Passagieren, Zellzahlbestimmung mittels Hamatozytometer und deren Kryokonservierung. ... Personen ... die sich nur mit einer speziellen Art von Zellen beschaftigen, ... finden auf dem Markt sicher geeignetere Literatur. Im Gegensatz hierzu ist ... ein gutes und ausfuhrliches Nachschlagewerk fur ... Wissenschaftler ... In deren Laboren durfte es eine sinnvolle Erganzung des Handapparates sein. (Stefan Jean-Pierre Haas, in: Annals of Anatomy, 2006, Vol. 188, S. 285 f.)

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