Overview

A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.

Full Product Details

Author:   Benny K. C. Lo
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   248
Dimensions:   Width: 15.50cm , Height: 3.10cm , Length: 23.50cm
Weight:   2.160kg
ISBN:  

9781588290922

ISBN 10:   1588290921
Pages:   562
Publication Date:   05 December 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Antibody Sequence And Structure.- Internet Resources for the Antibody Engineer.- The Kabat Database and a Bioinformatics Example.- IMGT, The International ImMunoGeneTics Information System®, http://imgt.cines.fr.- Antibody Variable Regions.- Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling.- Antibody-Lead Generation.- PCR Cloning of Human Immunoglobulin Genes.- Antibody Humanization by CDR Grafting.- Selection of Human Antibodies from Phage Display Libraries.- Production of Human Single-Chain Antibodies by Ribosome Display.- Production of Human Antibodies from Transgenic Mice.- Selection of Internalizing Antibodies for Drug Delivery.- Engineering Multivalent Antibody Fragments for In Vivo Targeting.- Production of Recombinant Bispecific Antibodies.- Antibody Expression And Optimization.- Expression and Isolation of Recombinant Antibody Fragments in E. coli.- Expression of Recombinant Antibodies in Mammalian Cell Lines.- Human Antibody Production Using Insect-Cell Expression Systems.- Antibody Production in Transgenic Plants.- Directed Mutagenesis of Antibody Variable Domains.- Antibody Affinity Maturation by Chain Shuffling.- Antibody Affinity Maturation by Random Mutagenesis.- Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting.- Antibody Characterization and Novel Applications.- Antibody Purification by Column Chromatography.- Affinity Measurement Using Surface Plasmon Resonance.- Kinetic Exclusion Assays to Study High-Affinity Binding Interactions in Homogeneous Solutions.- Characterization of Antibody-Antigen Interactions by Fluorescence Spectroscopy.- Antibody Epitope Mapping Using Arrays of Synthetic Peptides.- Mapping Antibody:Antigen Interactions by Mass Spectrometry and Bioinformatics.- Radiometal Labeling ofAntibodies and Antibody Fragments for Imaging and Therapy.- Production and Characterization of Anti-Cocaine Catalytic Antibodies.- Recombinant Immunotoxins in the Treatment of Cancer.- Antibodies in Proteomics.- Targeting of Antibodies Using Aptamers.

Reviews

...as the book provides as comprehensive collection of clear, detailed methods it is very likely to prove its worth as a laboratory reference. - Immunology News ...a valuable addition to in-lab books - Microbiology Today

...as the book provides as comprehensive collection of clear, detailed methods it is very likely to prove its worth as a laboratory reference. - Immunology News ...a valuable addition to in-lab books - Microbiology Today

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