A Practical Introduction to DNA Fingerprinting

Author:   D.A. Galbraith
Publisher:   Chapman and Hall
ISBN:  

9780412434105


Pages:   224
Publication Date:   November 1996
Format:   Hardback
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

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A Practical Introduction to DNA Fingerprinting


Overview

This is a practical guide to the use of DNA fingerprinting and related techniques of molecular genetics for applications in the biological sciences. It covers the theoretical background, planning, methodology, implementation, and analysis of DNA profiles for applications such as paternity analysis and analysis of relatedness in field populations of vertebrates. Written especially for scientists with no background in molecular genetics but who are interested in wildlife biology, conservation, demographics and evolutionary and behavioural ecology, this book bridges the gap between the specialized molecular techniques and their practical applications in ecological and evolutionary studies. It is a step-by-step guide combining training exercises, hands-on protocols, and detailed background information to give the researcher a guided introduction to the actual use of DNA fingerprinting in her own applications. This book should be of interest to wildlife biologists; conservation biologists; ecologists; population biologists; geneticists and other molecular biologists; and forensic scientists.

Full Product Details

Author:   D.A. Galbraith
Publisher:   Chapman and Hall
Imprint:   Chapman and Hall
Weight:   0.333kg
ISBN:  

9780412434105


ISBN 10:   0412434105
Pages:   224
Publication Date:   November 1996
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

The history and theory of DNA fingerprinting. Elements of experimental design for DNA fingerprinting. Genomes, genetics, and molecular methods. The technique of DNA fingerprinting. Laboratory safety. Fundamental laboratory skills. Organizing and equipping the laboratory. Laboratory protocols for DNA fingerprinting. Introduction to the protocol chapters. A lambdaDNA model for southern blots. Extraction and preparation of nuclear DNA. Agarose gel electrophoresis. Southern blotting. Solid base DNA hybridization of labelled probes. Interpretation and scoring of autoradiograms. Analysis of DNA fingerprint data. Epiolgue: whither DNA fingerprinting?

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