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Author:   Wei Xiao
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 3rd ed. 2014
Volume:   1163
Weight:   6.105kg
ISBN:  

9781493942770

ISBN 10:   1493942778
Pages:   313
Publication Date:   17 September 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Basic Investigations in Saccharomyces cerevisiae.- Isolation of Yeast Nucleic Acids.- Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry.- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.- Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe.- See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry.- Yeast Two Hybrid Liquid Screening.- Targeted Mutagenesis of a Specific Gene in Yeast.- Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae.- Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy.- Assays for Autophagy II: Mitochondrial Autophagy.- Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction.- Yeast Survival and Growth Assays.- Spontaneous Mutagenesis Assay.- Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene.- piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe.- Replicative and Chronological Life Span Assays.- Detection of Protein Arginine Methylation in Saccharomyces cerevisiae.- Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae.- Ligand Engineering Using Yeast Surface Display.- Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay.

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