Yeast Cytokinesis: Methods and Protocols

Author:   Alberto Sanchez-Diaz ,  Pilar Perez
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2016
Volume:   1369
ISBN:  

9781493979899


Pages:   395
Publication Date:   30 March 2019
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Yeast Cytokinesis: Methods and Protocols


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Author:   Alberto Sanchez-Diaz ,  Pilar Perez
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2016
Volume:   1369
Weight:   1.048kg
ISBN:  

9781493979899


ISBN 10:   1493979892
Pages:   395
Publication Date:   30 March 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Time-lapse Fluorescence Microscopy Of Budding Yeast Cells.- Real-time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells.- Fluorescence Recovery After Photo-bleaching (FRAP) and Fluorescence Loss in Photo-bleaching (FLIP) Experiments to Study Protein Dynamics during Budding Yeast Cell Division.- High-Speed Super Resolution Imaging of Live Fission Yeast Cells.- Monitoring Chitin Deposition during Septum Assembly in Budding Yeast.- Imaging Septum Formation by Fluorescence Microscopy.- Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy.- Visualization of Fission Yeast Cells By Transmission Electron Microscopy.- Characterization of Septin Ultrastructure in Budding Yeast Using Electron Tomography.- Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe.- Measurements of Myosin-II motor Activity during Cytokinesis In Fission Yeast.- In Vitro Biochemical Characterization of Cytokinesis Actin Binding Proteins.- Characterization of Cytokinetic F-BARs and other Membrane Binding Proteins.- Analysis of Three-Dimensional Structures of Exocyst Components.- Analysis of Rho-GTPase Activity during Budding-yeast Cytokinesis.- Detection of Phosphorylation Status of Cytokinetic Components.- Studying Protein-Protein Interactions in Budding Yeast Using Co-Immunoprecipitation.- Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening Of Suppressor Genes.- Synchronization of the Budding Yeast Saccharomyces cerevisiae.- Fission Yeast Cell Cycle Synchronization Methods.- A Review of Fluorescent Proteins for Use in Yeast.- Visualisation and Image Analysis of Yeast Cells.- Toolbox for Protein Structure Prediction.- From Structure to Function. A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand their Role in Cytokinesis.

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