Overview

This volume provides a collection of protocols for the most common experimental methods used for engineering Yarrowia lipolytica. Chapters detail the basic theories underlying the methods described in each chapter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Yarrowia lipolytica: Methods and Protocols aims to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully.

Full Product Details

Author:   Ian Wheeldon ,  Mark Blenner
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2307
Weight:   0.500kg
ISBN:  

9781071614167

ISBN 10:   1071614169
Pages:   250
Publication Date:   15 April 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1.                  Genome Engineering of Yarrowia lipolytica with the piggyBac Transposon System James M. Wagner, Claire M. Palmer, Maya V. Venkataraman, Lars H. Lauffer, Joshua M. Wiggers, Eden V. Williams, Xiunan Yi, and Hal S. Alper   2.                  Genome Editing in Y. lipolytica Using TALENs Coraline Rigouin, Christian Croux, Gwendoline Dubois, Fayza Daboussi, and Florence Bordes   3.                  EasyCloneYALI: Toolbox for CRISPR-mediated Integrations and Deletions in Yarrowia lipolytica Jonathan Dahlin, Carina Holkenbrink, and Irina Borodina   4.                  Simultaneous gene excision and integration by dual-guide CRISPR-Cas9 Michael Spagnuolo and Mark Blenner   5.                  Gene excision by dual-guide CRISPR-Cas9 Michael Spagnuolo and Mark Blenner   6.                  CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica Joshua Misa and Cory Schwartz   7.                  Implementing CRISPR-Cas12a for Efficient Genome-editing in Yarrowia lipolytica Zhiliang Yang and Peng Xu   8.                  Guide RNA Design for Genome-wide CRISPR Screens in Yarrowia lipolytica Adithya Ramesh and Ian Wheeldon   9.                  Targeted Integration through Transformation of Hydroxyurea-Arrested Cells Vasiliki Tsakraklides   10.              Strain Construction for Intracellular Metabolic Pathway Localization in Y. lipolytica Erin L. Bredeweg and Scott E. Baker   11.              Title: Engineering Yarrowia lipolytica for production of fatty alcohols with YaliBrick Vectors Wanqi Sun, Zhiliang Yang and Peng Xu   12.              Methods to Activate and Elucidate Complex Endogenous Sugar Metabolism in Yarrowia lipolytica Seunghyun Ryu and Cong T. Trinh   13.              Pathway Engineering For Beta-Carotene and Carotenoid Biosynthesis in Y. lipolytica Mauricio Pesantes-Munoz and Rodrigo Ledesma-Amaro   14.              Droplet-based Microfluidic High-throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica Thomas Beneyton and Tristan Rossignol   15.              Yarrowia lipolytica Bioprocess Development – From Flask to Bioreactor Farshad Darvishi and Marzieh Moradi   16.              Western Blotting of Membrane-Bound Proteins in Yarrowia lipolytica Adithya Ramesh and Ian Wheeldon

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