Overview

During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of  Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.  

Full Product Details

Author:   STEFAN HOPPLER ,  Peter D Vize
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2012
Volume:   917
Dimensions:   Width: 17.80cm , Height: 3.60cm , Length: 25.40cm
Weight:   1.281kg
ISBN:  

9781617799914

ISBN 10:   1617799912
Pages:   568
Publication Date:   06 September 2012
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Not yet available  
This item is yet to be released. You can pre-order this item and we will dispatch it to you upon its release.

Table of Contents

Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present and Future.-Husbandry of Xenopus tropicalis.-Generating Diploid Embryos from Xenopus tropicalis.-Navigating the Xenopus tropicalis Genome.-Genetic Analysis of Xenopus tropicalis.-Forward Genetic Screens in Xenopus using Transposon-mediated Insertional Mutagenesis.-Targeted Gene Disruption with Engineered Zinc-finger Nucleases (ZFNs).-Reverse Genetic Studies using Antisense Morpholino Oligonucleotides.-Chemical Genetics and Drug Discovery in Xenopus.-Maternal mRNA Knock-down Studies: Antisense Experiments using the Host-transfer Technique in Xenopus laevis and Xenopus tropicalis.-Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI).-A Simple Method of Transgenesis using I-SceI meganuclease in Xenopus.-Using FC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos.-Xenopus Transgenics: Methods using Transposons.-Comparative Genomics-based Identification and Analysis of Cis-regulatory Elements.-Tet-On Binary Systems for Tissue-specific and Inducible Transgene Expression.-Chromatin Immunoprecipitation Analysis of Xenopus Embryos.-Transcriptomics Using Next Generation Sequencing Technologies.-Databases of Gene Expression in Xenopus Development.-Investigating Alternative RNA Splicing in Xenopus.-Immunoisolation of Protein Complexes from Xenopus.-Complementary Proteomic Analysis of Protein Complexes.-Antibody Development and use in Chromogenic and Fluorescent Immunostaining.-Multiple fluorescent  in situ mRNA Hybridization (FISH) on Whole Mounts and Sections.-Methods to Analyze microRNA Expression and Function During Xenopus Development.-A Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos.-Microscopy Tools for Quantifying Developmental Dynamics in XenopusEmbryos.-Mathematical Modelling of Gene RegulatoryNetworks in Xenopus Development.-Stem-cell-like Embryonic Explants to Study Cardiac Development.-Studying Regeneration in Xenopus.-On-line Resources for Xenopus.Mathematical Modelling of Gene RegulatoryNetworks in Xenopus Development.-Stem-cell-like Embryonic Explants to Study Cardiac Development.-Studying Regeneration in Xenopus.-On-line Resources for Xenopus.

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