Viral Metagenomics: Methods and Protocols

Author:   Vitantonio Pantaleo ,  Laura Miozzi
Publisher:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2024
Volume:   2732
ISBN:  

9781071635148


Pages:   288
Publication Date:   08 December 2023
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Viral Metagenomics: Methods and Protocols


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Overview

This second edition volume expands on the previous edition with discussions about the latest viral metagenomics aspects covering a range of different specimens such as soil, freshwater, wastewater, fecal samples, blood plasma, clinical tissues, fungi, and herbarium samples. Chapters also look at different viral groups including archaeal viruses, eukaryotic viruses, phages, mycoviruses, and circular DNA viruses. Techniques required for studying the three viral metagenomic steps of samples processing, library construction, and analysis of data are also discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Vital Metagenomics: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this important and developing field.

Full Product Details

Author:   Vitantonio Pantaleo ,  Laura Miozzi
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2024
Volume:   2732
ISBN:  

9781071635148


ISBN 10:   107163514
Pages:   288
Publication Date:   08 December 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Exploring the Archaeal Virosphere by Metagenomics.- From Whole-Genome Shotgun Sequencing to Viral Community Profiling: The ViromeScan Tool.- Freshwater Viral Metagenome Analyses Targeting dsDNA Viruses.- Discovery and Identification of Viruses Infecting Oomycetes.- Metagenomic Analyses of Viruses in the Orchid Mycorrhizal Interaction using Improved Assemble Tools.- Determination of the Mycovirome of a Necrotrophic Fungus.- From Metagenomics to Ecogenomics: NGS-Based Approaches for Discovery of New Circular DNA Single-Stranded Viral Species.- SARS-CoV-2 Detection and Genome Sequencing in Urban Wastewaters.- Enrichment, Sequencing, and Identification of DNA Bacteriophages from Fecal Samples.- Exploration and Retrieval of Virus-Related Molecular Data using ExTaxsI: The Monkeypox Use Case.- Optimized Recovery of Viral DNA and RNA from Blood Plasma for Viral Metagenomics.- Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.- Viromes of Plants Determined by High-Throughput Sequencing of Virus Derived siRNAs.- Validation of High-Throughput Sequencing (HTS) for Routine Detection of Citrus Viruses and Viroids.- Identification of DNA Viruses in Ancient DNA from Herbarium Samples.- The Oxford Nanopore MinION as a Versatile Technology for the Diagnosis and Characterization of Emerging Plant Viruses.- Detection of Plant Viruses using Nanopore Sequencing Based Metagenomic Approach.- Application of Nanopore-Based Sequencing to Identify Virus Infections in Woody Plants.- Distribution of Small RNAs Along Transposable Elements in Vitis vinifera during Somatic Embryogenesis.

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