Overview

This second edition includes a collection of antigen production and delivery strategies for vaccine development in veterinary species. New and updated chapters guide readers through protocols for antigen production, experimental antigen delivery and the analysis of immune responses upon vaccination. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.  Authoritative and cutting-edge, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols, Second Edition aims to be a useful and practical guide to researches to help further their study in this field. 

Full Product Details

Author:   Alejandro Brun
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2465
Weight:   0.610kg
ISBN:  

9781071621707

ISBN 10:   107162170
Pages:   306
Publication Date:   04 February 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. An overview of veterinary viral diseases and vaccine technologies Alejandro Brun   2 .Production and purification of candidate subunit vaccines by IC-tagging protein encapsulation Natalia Barreiro-Piñeiro; Tomás Pose Boirazian; Rebeca Menaya-Varga,  and José M. Martínez-Costas.   3. Elastin‐like polymers as nanovaccines: protein engineering of self‐assembled, epitope-exposing nanoparticles Alessandra Girotti, Juan González‐Valdivieso, Irene Alonso‐Sampedro, Sara Escalera-Anzola, Sandra Ramos‐Díez, and  F. Javier Arias   4. Display of heterologous proteins in Bacillus subtilis biofilms for enteric immunization Ramona Wissmann, Cornel Fraefel, Claudio Aguilar, and Catherine Eichwald   5. Production of influenza H5 vaccine oligomers in plants Hoang Trong Phan and Udo Conrad   Chapter 6 DNA vaccines in pigs: from immunization to antigen identification. Francesc Accensi, Laia Bosch-Camós, Paula L. Monteagudo and Fernando Rodríguez   7. Use of foot-and-mouth disease virus non-coding synthetic RNAs as vaccine adjuvants Miguel Rodríguez-Pulido, Miryam Polo, Belén Borrego, and Margarita Sáiz   8. Evaluation of innate lymphoid cells and dendritic cells following viral vector vaccination. Zheyi Li, Sreeja Roy, and Charani Ranasinghe   9. Generation of replication deficient human adenovirus 5 (Ad5) vectored FMD vaccines. Gisselle N. Medina, Teresa de los Santos, and Fayna Diaz-San Segundo   10. Recombinant modified vaccinia virus Ankara development to express VP2, NS1, and VP7 proteins of Bluetongue virus Alejandro Marín-López, Sergio Utrilla-Trigo, Luis Jiménez-Cabello, and Javier Ortego.   11. Cloning strategies for the generation of recombinant capripoxvirus through the use of screening and selection markers Hani Boshra, Mahder Teffera, Jinxing Cao, and Shawn Babiuk   12. Using Rift Valley fever virus as a vector platform for the expression of ruminant disease antigens Sandra Moreno Belén Borrego and Alejandro Brun    13. Generation and characterization of single-cycle infectious canine Influenza A Virus (sciCIV) and its use as vaccine platform Aitor Nogales, Kevin Chiem, Michael Breen, Marta L DeDiego, Colin R Parrish, and Luis Martínez-Sobrido   14. Reverse genetics for influenza A and B viruses driven by swine polymerase I promoter. Brittany Seibert, Stivalis Cardenas-Garcia, Daniela Rajao, and Daniel R. Perez   15. Analysis of the cellular immune responses to vaccines Nicholas Svitek, Evans L. N. Taracha, Rosemary Saya, Elias Awino, Vish Nene, and Lucilla Steinaa  

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