Overview

This volume is provides the tools for investigators to learn how to manipulate the ubiqutin-proteasome system to address questions in their own areas of reaserch. This borad field is covered in detail from basic biochemistry to cellular assays to discovery techniques using mass spectroscopic analysis. It will serve as a useful tool to accelerate discovery and enhance productivity.

Full Product Details

Author:   Cam Patterson ,  Douglas M. Cyr
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2005 ed.
Volume:   301
Dimensions:   Width: 15.50cm , Height: 2.20cm , Length: 23.50cm
Weight:   0.730kg
ISBN:  

9781588292520

ISBN 10:   1588292525
Pages:   381
Publication Date:   01 April 2005
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Biochemical Methods to Study the Ubiquitin-Proteasome System.- Small-Molecule Inhibitors of Proteasome Activity.- Purification of E1 and E1-Like Enzymes.- Assays for RING Family Ubiquitin Ligases.- Ubiquitin Chain Synthesis.- Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Budding Yeast.- Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome.- Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation.- Assays of Proteasome-Dependent Cleavage Products.- Identification of Components of Protein Complexes.- Mass Spectrometric Determination of Protein Ubiquitination.- Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol.- Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation.- Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization.- Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions.- Measuring Ubiquitin Conjugation in Cells.- Assays for Proteasome Assembly and Maturation.- N-Terminal Ubiquitination.- Quantitating Defective Ribosome Products.- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation.- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation.- Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells.- Aggresome Formation.- Detection of Sumoylated Proteins.- Proteasome Inhibitors in Cancer Therapy.- Parkinson’s Disease.

Reviews

.,. clearly written and thorough...a valuable addition to any lab entering, or already working in, the ubiquitin or proteasome field. - Doody's Health Science Book Review Journal

...clearly written and thorough...a valuable addition to any lab entering, or already working in, the ubiquitin or proteasome field. - Doody's Health Science Book Review Journal

"""...clearly written and thorough...a valuable addition to any lab entering, or already working in, the ubiquitin or proteasome field."" - Doody's Health Science Book Review Journal"

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