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Author:   Claire Stocker
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2009 ed.
Volume:   560
Dimensions:   Width: 19.30cm , Height: 2.30cm , Length: 26.00cm
Weight:   0.897kg
ISBN:  

9781934115152

ISBN 10:   1934115150
Pages:   278
Publication Date:   10 July 2009
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Selecting the “Right” Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research.- Nutritional Models of Type 2 Diabetes Mellitus.- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans.- The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans.- The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ?-Cell Lines.- In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function.- Single-Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells.- Laser Capture Microdissection of Human Pancreatic ?-Cells and RNA Preparation for Gene Expression Profiling.- In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells.- The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes.- Pancreatic Remodeling: Beta-Cell Apoptosis, Proliferation and Neogenesis, and the Measurement of Beta-Cell Mass and of Individual Beta-Cell Size.- Morphology of Pancreatic Islets: A Time Course of Pre-diabetes in Zucker Fatty Rats.- Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas.- The Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent.- Hyperinsulinemic–Euglycemic Clamp to Assess Insulin Sensitivity In Vivo.- Gene Expression Analysis in Diabetes Research.- Gene Expression Mining in Type 2 Diabetes Research.

Reviews

From the reviews: This book provides in 17 chapters laboratory protocols used in diabetes research. It describes methods using new technologies in studying pancreatic beta cells. For research diabetologists. (Pediatric Endocrinology Reviews, Vol. 7 (4), June, 2010)

From the reviews: This book provides in 17 chapters laboratory protocols used in diabetes research. It describes methods using new technologies in studying pancreatic beta cells. For research diabetologists. (Pediatric Endocrinology Reviews, Vol. 7 (4), June, 2010)

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