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OverviewFull Product DetailsAuthor: David Sheehan , Raymond TytherPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of hardcover 1st ed. 2009 Volume: 519 Weight: 1.364kg ISBN: 9781617378492ISBN 10: 1617378496 Pages: 546 Publication Date: 19 November 2010 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand ![]() We will order this item for you from a manufactured on demand supplier. Table of ContentsTwo-Dimensional Electrophoresis: An Overview.- Solubilization of Proteins in 2DE: An Outline.- Selection of pH Ranges in 2DE.- Difficult Proteins.- Organelle Proteomics.- Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry.- Immunoblotting 2DE Membranes.- Troubleshooting Image Analysis in 2DE.- Analysis of Bacterial Proteins by 2DE.- Proteomic Analysis of Caenorhabditis elegans.- Protein Extraction for 2DE.- Analysis of Proteins from Marine Molluscs.- Preparation and Analysis of Plant and Plastid Proteomes by 2DE..- High-Resolution 2DE.- Blue Native-Gel Electrophoresis Proteomics.- 2DE for Proteome Analysis of Human Metaphase Chromosomes.- Microsomal Proteomics.- Prefractionation Using Microscale Solution IEF.- Diagonal Electrophoresis for Detection of Protein Disulphide Bridges.- High-Resolution Large-Gel 2DE.- Silver Staining of Proteins in 2DE Gels.- Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach.- Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells.- Detection of Ubiquitination in 2DE.- Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry.- Detection of Protein Glutathionylation.- Activity-Based Protein Profiling of Protein Tyrosine Phosphatases.- Active Protease Mapping in 2DE Gels.- Two-Dimensional Difference Gel Electrophoresis.- Protein Expression Profiling.- C-Terminal Sequence Analysis of 2DE-Separated Proteins.- Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry.- de Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination.- Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis.- Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations.- Creating 2DE Databases for the World Wide Web.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |