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Author:   David Sheehan ,  Raymond Tyther
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2009
Volume:   519
Weight:   1.364kg
ISBN:  

9781617378492

ISBN 10:   1617378496
Pages:   546
Publication Date:   19 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Two-Dimensional Electrophoresis: An Overview.- Solubilization of Proteins in 2DE: An Outline.- Selection of pH Ranges in 2DE.- Difficult Proteins.- Organelle Proteomics.- Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry.- Immunoblotting 2DE Membranes.- Troubleshooting Image Analysis in 2DE.- Analysis of Bacterial Proteins by 2DE.- Proteomic Analysis of Caenorhabditis elegans.- Protein Extraction for 2DE.- Analysis of Proteins from Marine Molluscs.- Preparation and Analysis of Plant and Plastid Proteomes by 2DE..- High-Resolution 2DE.- Blue Native-Gel Electrophoresis Proteomics.- 2DE for Proteome Analysis of Human Metaphase Chromosomes.- Microsomal Proteomics.- Prefractionation Using Microscale Solution IEF.- Diagonal Electrophoresis for Detection of Protein Disulphide Bridges.- High-Resolution Large-Gel 2DE.- Silver Staining of Proteins in 2DE Gels.- Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach.- Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells.- Detection of Ubiquitination in 2DE.- Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry.- Detection of Protein Glutathionylation.- Activity-Based Protein Profiling of Protein Tyrosine Phosphatases.- Active Protease Mapping in 2DE Gels.- Two-Dimensional Difference Gel Electrophoresis.- Protein Expression Profiling.- C-Terminal Sequence Analysis of 2DE-Separated Proteins.- Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry.- de Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination.- Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis.- Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations.- Creating 2DE Databases for the World Wide Web.

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