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Author:   Sandeep Raha
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2024
Volume:   2728
ISBN:  

9781071634943

ISBN 10:   1071634941
Pages:   248
Publication Date:   30 November 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Isolation and Maintenance in Culture of Primary Human Trophoblast from term Placentae.- Isolation of Human Villous Cytotrophoblastic Cells from Term Placenta for Transfection.- Enzymatic Digestion and Single Cell Isolation of Peri-Implantation Stage Human Trophoblast Cells.- Precision-Cut Slice Culture Method for Rat Placenta.- Evaluation of Molecular Interactions and Cellular Dynamics at the Maternal-Fetal Interface during Placental Morphogenesis.- In Vivo Quantitative Assessment of Gestational Choriocarcinoma Development and Progression using Luminescent Trophoblast Cells.- Analyzing Trophoblast Fusion using Immunofluorescence and Split Protein Complementation Assays.- Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling.- Assessment of Placental Sodium Independent Leucine Uptake and Transfer in Trophoblast Cells.- Assessing Cholesterol Efflux on Primary Human Trophoblast Cells.- RGD-Based Fluorescenceto Assess Placental Angiogenesis.- Real-Time Assessment of Mitochondrial Function in Cytotrophoblast and Syncytialized Trophoblast Cells using the Seahorse XFe24 Extracellular Flux Analyzer.- Quantifying Trophoblast Function using the xCELLigence System.- Liposome Encapsulated Anti-Inflammatory Proteins for targeted Delivery to the Placenta to Treat Fetal Growth Restriction.- Trophoblast-Targeted Lipsomes for Placenta-Specific Drug Delivery.- Methods for Co-Culture of Primary Human Extravillous Trophoblast Cells and Uterine Natural Killer Cells.- Trophoblast Organoids as a Novel Tool to Study Human Placental Development and Function.- A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening.- Three-Dimensional In Vitro Human Placental Organoids from Mononuclear Villous Trophoblasts or Trophoblast Stem Cells to Understand Trophoblast Dysfunction in Fetal Growth Restriction.

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