Overview

This detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus Trichoderma reesei. Beginning with overviews of its evolution, its use in the production of industrially-relevant enzymes, and synthetic biology applications, the volume continues with methods covering topics from transformation techniques and gene editing to downstream-analytical applications and -omics analyses and the corresponding bioinformatics approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Trichoderma reesei: Methods and Protocols serves as an ideal guide for a wide range of audiences, from students who want to familiarize themselves with basic research protocols to experienced scientists who are planning to establish a new method in their laboratories, working with this dynamic fungus.

Full Product Details

Author:   Astrid R. Mach-Aigner ,  Roland Martzy
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2234
Weight:   0.903kg
ISBN:  

9781071610473

ISBN 10:   1071610473
Pages:   364
Publication Date:   10 November 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Ecological Genomics and Evolution of Trichoderma reesei.- Industrial Relevance of Trichoderma reesei as an Enzyme Producer.- The Potential of Synthetic Biology for Trichoderma reesei.- Resistance Marker- and Gene Gun-Mediated Transformation of Trichoderma reesei.- Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei.- Open the Pores: Electroporation for the Transformation of Trichoderma reesei.- Sexual Crossing of Trichoderma reesei.- CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei.- The Copper-Controlled RNA Interference System in Trichoderma reesei.- Batch Cultivation of Trichoderma reesei.- Image Analysis Method for the Characterization of Trichoderma reesei during Fermentations.- Measurement of Cellulase and Xylanase Activities in Trichoderma reesei.-  Flow Cytometry for Filamentous Fungi.- Molecular Identification of Trichoderma reesei.-In Vivo Footprinting Analysis in Trichoderma reesei.- RNA Characterization in Trichoderma reesei.- Proteomic Profiling of the Secretome of Trichoderma reesei.- Transcriptomics in Trichoderma reesei.- The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances.- In Silico Gene Analysis and Oligonucleotide Design for Construction of Expression Vectors.- PacBio Long-Read Sequencing, Assembly, and Funannotate Reannotation of the Complete Genome of Trichoderma reesei QM6a.- TSETA: A Third-Generation Sequencing-Based Computational Tool for Mapping and Visualization of SNPs, Meiotic Recombination Products, and RIP Mutations.

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