Overview

This volume focuses on procedures for the development and application of several research animal models and in vitro methods that allow researchers to gain insight into the underlying cellular, biochemical, and physiological mechanisms involved in traumatic and ischemic injury. The chapters in this book discuss topics, such as animal models and techniques commonly used to study traumatic brain injury, hemorrhagic shock, ischemic and hemorrhagic strokes, sepsis, burn injury, hind limb ischemia, myocardial-ischemia-reperfusion injury, intracranial pressure, global hypoxia-induced perinatal seizures models, and in vitro models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Traumatic and Ischemic Injury: Methods and Protocols is a valuable resource for novices with limited experience to help them initiate new research projects, and established researchers to help them identify comparable approaches and strategies to their studies in this field.

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9781493985104

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