Transgenic Animal Technology: A Laboratory Handbook

Author:   Carl A. Pinkert (Professor of Pathobiology, Auburn University, AL, USA)
Publisher:   Elsevier Science Publishing Co Inc
Edition:   2nd edition
ISBN:  

9780125571661


Pages:   618
Publication Date:   04 December 2002
Replaced By:   9780124104907
Format:   Hardback
Availability:   In Print   Availability explained
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

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Transgenic Animal Technology: A Laboratory Handbook


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Full Product Details

Author:   Carl A. Pinkert (Professor of Pathobiology, Auburn University, AL, USA)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Edition:   2nd edition
Dimensions:   Width: 19.10cm , Height: 2.80cm , Length: 23.50cm
Weight:   1.070kg
ISBN:  

9780125571661


ISBN 10:   0125571666
Pages:   618
Publication Date:   04 December 2002
Audience:   Professional and scholarly ,  Professional & Vocational
Replaced By:   9780124104907
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   In Print   Availability explained
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Section One Overview. Introduction to transgenic animals. Section Two Transgenic animal production focusing on the mouse model. Transgenic animal production using DNA microinjection. Factors affecting transgenic animal production. Gene targeting in embryonic stem cells I: History and methodology. Gene targeting in embryonic stem cells II. Conditional technologies. Retrovirus-mediated gene transfer. Nuclear transfer technologies. Section Three Production of transgenic laboratory and domestic animal species. Production of transgenic rats. Production of transgenic rabbits. Production of transgenic fish. Production of transgenic poultry. Production of transgenic swine by DNA Microinjection. Production of transgenic ruminants by DNA Microinjection. Production of transgenic non-human primates. Retrovirus-mediated gene transfer into oocytes. Section Four: Molecular biology, analyses and enabling technologies Vector design for Transgene Expression. Analysis of transgene integration. PCR optimization for Detection of Transgenic Integration. Evaluation of Transgene Expression. Control of gene expression (from standard gain/loss of function to anti-sense to PNAs). Enabling technologies:Cryopreservation of gametes. Enabling technologies:Embryo culture considerations. Databases, Internet Resources and Genetic Nomenclature.

Reviews

...this edition covers an exceptionally wide range of aspects of transgenic technology...The major strength of the book is that it comprehensively covers most important aspects of transgenic technology. Carl Pinkert must be congratulated on bringing together a set of expert authors who have covered all essential aspects of transgenesis. A 'must have' for every lab involved with transgenic technology. -Andreas Kolb, Hannah Research Institute The handbook provides an excellent distillation of practical aspects of the fast growing field of animal transgenesis...the second edition contains substantial new and updated information...chapters are well illustrated with useful figures supporting the text...the handbook will be a valuable resource for two audiences, one seeking a broad appreciation for transgenic animal technology, which can be gained through reading the handbook cover to cover, and the other seeking concise descriptions of focused techniques which can be found by selection of specific chapters...overall, the text is well edited...$75.00 and its approximately 600 pages are worth the cost. -TRANSGENICS (2003) This handbook would be valuable to the investigators producing transgenic animal models. It is well organized and the handbook provides a thorough description of current animal transgenic technology across a host of animals. -ANALYTICAL BIOCHEMISTRY (2003)


...this edition covers an exceptionally wide range of aspects of transgenic technology...The major strength of the book is that it comprehensively covers most important aspects of transgenic technology. Carl Pinkert must be congratulated on bringing together a set of expert authors who have covered all essential aspects of transgenesis. A 'must have' for every lab involved with transgenic technology. -Andreas Kolb, Hannah Research Institute The handbook provides an excellent distillation of practical aspects of the fast growing field of animal transgenesis...the second edition contains substantial new and updated information...chapters are well illustrated with useful figures supporting the text...the handbook will be a valuable resource for two audiences, one seeking a broad appreciation for transgenic animal technology, which can be gained through reading the handbook cover to cover, and the other seeking concise descriptions of focused techniques which can be found by selection of specific chapters...overall, the text is well edited...$75.00 and its approximately 600 pages are worth the cost. -TRANSGENICS (2003) This handbook would be valuable to the investigators producing transgenic animal models. It is well organized and the handbook provides a thorough description of current animal transgenic technology across a host of animals. -ANALYTICAL BIOCHEMISTRY (2003)


A `must have' for every lab involved with transgenic technology. -Andreas Kolb, Hannah Research Institute The handbook will be a valuable resource for two audiences, one seeking a broad appreciation for transgenic animal technology, which can be gained by reading the handbook cover to cover, and the other seeking concise descriptions of focused techniques, which can be found by selection of specific chapters. -TRANSGENICS (2003) This handbook would be valuable to the investigators producing transgenic animal models. It is well organized and the handbook provides a thorough description of current animal transgenic technology across a host of animals. -ANALYTICAL BIOCHEMISTRY (2003)


Author Information

Dr. Pinkert is an Associate Professor in the Department of Comparative Medicine and Director of the Transgenic Animal/ES Cell Resource at the University of Alabama at Birmingham. He earned his B.S. from Colorado State University, M.S. from Southern Illinois, and Ph.D. in 1983 from the University of Georgia, followed by postdoctoral training at the University of Pennsylvania. He was an Assistant Professor at the University of Missouri and then worked at DNX, Inc. (where his proposal for the National Transgenic Development Facility for the National Institute of Child Health and Human Development was awarded and implemented) before joining the UAB faculty. Dr. Pinkert's primary research efforts involve gene transfer, expression, and regulation using transgenic animal modeling. His work has illustrated the potential of modifying the immune system, enhancing growth performance, and the feasibility of molecular farming. He has published extensively on transgenic modeling, reproductive biology, and embryology of laboratory and domestic animals. He serves as an Editro of Transgenic Research and has served as an ad hoc reviewer or consultant for several journals, government agencies, and companies.

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